Figure 4.
Quantifying in utero protein turnover across mouse fetal development
(A) Experimental design for quantifying protein turnover. Fetuses from E13.5, E14.5, and E16.5 are pulse injected with labeled amino acids. P0 mice are pulse injected through the retro-orbital vein.36 Fetal liver and lung tissues are harvested from different pregnant mice at 2, 4, and 6 h post-injection and analyzed by mass spectrometry.
(B) Distribution of batch-corrected and normalized log2-transformed peptide abundances. MS runs were batch corrected using a common standard protein sample that was processed with every batch.
(C) UMAP dimensionality reduction of the liver and lung proteomes from all time points.
(D) Turnover rates of hemoglobin proteins in the liver at different gestational ages.
(E and F) Quantified peptide turnover rates for different proteins at defined gestational stages from the liver and lung, respectively.