Table 1.

The limit of detection (LOD) in monoplex and triplex formats

Sample	Virus	LOD (Copy Number)
		Conventional RT-PCR	Monoplex MAGPIX assay	Triplex MAGPIX assay	Real-time RT-PCR
1	Bovine rotavirus	1.10 × 105	1.62 × 105	2.43 × 105	5.00
2	Bovine coronavirus	1.61 × 104	2.91 × 104	4.87 × 104	4.00
3	Bluetongue virus	2.26 × 105	3.74 × 105	2.13 × 105	5.00