TABLE 1.
Oligonucleotide primers and probes used in this study
| Primer or probe class | Name | Sequencea |
|---|---|---|
| VH family specific primers | 7183CDR1 | 5′-ATGTCTTGGGTTCG-3′ |
| 7183CDR2 | 5′-GGTGAATCGGCCCTT-3′ | |
| Q52CDR1 | 5′-AGCTATGGTGTACAC-3′ | |
| Q52CDR2 | 5′-TATGAAAGCTGCATT | |
| S107CDR1 | 5′-CTTCTGGGTTCACCT-3′ | |
| S107CDR2 | 5′-AGATGCATCGTACTC-3′ | |
| SM7CDR1 | 5′-GACTACTATATGCAC-3′ | |
| SM7CDR2 | 5′-CTGGAACTTCGGGTC-3′ | |
| 3660CDR1 | 5′-TACTGGAACTGGATC-3′ | |
| 3660CDR2 | 5′-CTTTTGAGAGATGGA-3′ | |
| V-Gam3/8CDR1 | 5′-CTATGGAATGAACTGG-3′ | |
| V-Gam3/8CDR2 | 5′-CAAACCGTCCCTTGAA-3′ | |
| J606CDR1 | 5′-AACTACTGGATGTCC-3′ | |
| J606CDR2 | 5′-AGACTCCGCATAATG-3′ | |
| V10CDR1 | 5′-ATACCAATGCCATGA-3′ | |
| V10CDR2 | 5′-CTGAATCGGCATAATA-3′ | |
| 3609CDR1 | 5′-ACTTCTGGTATGGGT-3′ | |
| 3609CDR2 | 5′-CAGGGCTGTGTTATA-3′ | |
| J558VaCDR1 | 5′-AACTACTGGGTAGGT-3′ | |
| J558VaCDR2 | 5′-CTTGAACTTCTCATT-3′ | |
| 7183CDR1gene spec. | 5′-TGCCATGTCTTGGGT-3′ | |
| VH1 5′ primers | 5′VH1a | 5′-GCCCAGAGTGACTCT-3′ |
| 5′VH1b | 5′-GGATCCTGTCCTGAG-3′ | |
| Primers for D-J and V-DJ PCR | 5′7183Fr3 | 5′-AAGAASAMCCTGTWCCTGCAATGASC-3′ |
| 5′558Fr3 | 5′-TCCARCACAGCCTWCATGCARCTCARC3′ | |
| 5′Q52Fr3 | 5′-AGACTGARCATCASCAAGGACAAYTCC-3′ | |
| 5′S107CDR2 | 5′-YAACAGAGTACAGTGCATCTRTKAAGGG-3′ | |
| 5′3609CDR2 | 5′-KCYYTGAAGAGCCRRCTCACAATCTCC-3′ | |
| 5′3660CDR2 | 5′-CTCAAAAGTCAATCTCCATCACTCGA-3′ | |
| 5′V-Gam3/8CDR2 | 5′-CAAGGGACGGTTTGCCTTCTCTTTGGAA-3′ | |
| 5′V10CDR2 | 5′-CGCATAAGCCGTAAAAGTARTAATTATGC-3′ | |
| 5′SM7CDR2 | 5′-GGATTGATYCTGMGAATGGTAATGCT-3′ | |
| DHL | 5′-GGAATTCGMTTTTTGTSAAGGGATCTACTACTGTG-3′ | |
| J3 | 5′-GTCTAGATTCTCACAAGAGTCCGATAGACCCTGG-3′ | |
| Probes for PCR products | ||
| 7183P | 5′-AAGAGGCTGGAGTGGGTC-3′ | |
| Q52p | 5′-GATATGGAGTGGTGGAAGCAC-3′ | |
| S107p | 5′-GCAAGTAGAAACAAAGCTAATG-3′ | |
| SM7p | 5′-AGGATTGATCCTGCGAATG-3′ | |
| 3660p | 5′-ATGGGGTACATAAGCTACAGT-3′ | |
| V-GAM3.8p | 5′-TGGATGGGCTGGATAAACAC-3′ | |
| J606p | 5′-GTTGCTCAAATAAGATTGAAATCT-3′ | |
| V10p | 5′-CGCATAAGAAGTAAAAGT-3′ | |
| 3609p | 5′-CTGGCACACATTTGGTGGGAT-3′ | |
| J558p | 5′-CTTGAGTGGATTGGAGATATT-3′ | |
| JH3 | 5′-CCTGGTTTGCTTACTGGG-3′ |
a
The sequences are presented 5′ to 3′. The degenerate nucleotide positions are coded as follows: R is A or G; Y is C or T; S is C or G; M is A or C; K is G or T; W is A or T; B is C, G, or T.