Fig. 4.

SUSP-4 improves inflammatory bowel disease (IBD) symptoms in mice. (A) Experimental process diagram. (B) Body weight change and disease activity index (DAI) score in mice. (C) Colonic tissue measurement. (D) Hematoxylin-eosin (H&E) staining of colon. (E) Immunofluorescence staining of the colon and Western blotting assay. Red was zonula occludens protein 1 (ZO-1) and green was occludin. (F) Serum biochemical analysis of myeloperoxidase (MPO), malondialdehyde (MDA), total-superoxide dismutase (T-SOD), catalase (CAT), interleukin-1β (IL-Iβ), IL-6, and tumor necrosis factor-α (TNF-α). Data are presented as the mean ± standard deviation (SD) (n = 8). ^∗P < 0.05 and ^∗∗P < 0.01, compared with the Model group. AUC: area under the curve; DAPI: 4',6-diamidino-2-phenylindole; GAPDH: glyceraldehyde-3-phosphate dehydrogenase.