TABLE 3.
Interaction between Snf1 and Mig1 in the two-hybrid systema
| LexA hybrid | GAD hybrid | β-Galactosidase activity (U/mg of protein)b
|
|
|---|---|---|---|
| 5% Glu | Shift to 0.05% Glu | ||
| Snf1 | GAD | 5 | 3 |
| Snf1K84R | GAD | 4 | 3 |
| Snf1 | Mig1ΔZ | 11 | 72 |
| Snf1K84R | Mig1ΔZ | 172 | 162 |
| Snf1 | Mig1ΔZS222*S278*S311* | 19 | 1,110 |
| Mig1ΔZS278*S311*S381* | 11 | 810 | |
| LexA | Mig1ΔZ | 9 | 6 |
| Mig1ΔZS222*S278*S311* | 6 | 5 | |
| Mig1ΔZS278*S311*S381* | 5 | 7 | |
a
Strain CTY10-5d was transformed with plasmids expressing the indicated proteins (see Materials and Methods). Immunoblot analysis showed that LexA-Snf1 and LexA-Snf1K84R are expressed at the same levels. GAD-Mig1ΔZ was used because it does not bind to DNA. Transformants were grown to exponential phase in selective SC medium plus 5% glucose (Glu) and then shifted to SC medium plus 0.05% glucose for 3 h.
b
β-Galactosidase activity was assayed in protein extracts. Values are averages for 3 to 16 transformants, and standard errors were <19%.