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. 1978 Sep;62(3):449–453. doi: 10.1104/pp.62.3.449

Effect of Gibberellin A3 on the Endoplasmic Reticulum and on the Formation of Glyoxysomes in the Endosperm of Germinating Castor Bean 1

Elma González 1
PMCID: PMC1092144  PMID: 16660535

Abstract

Seedlings of castor bean (Ricinus communis cv. Hale) were exposed to a range of concentrations of gibberellin A3 (GA3). Treatments for 20 hours with GA3 concentrations of 0.5 μM or higher resulted in increased levels of NADH-cytochrome c reductase, phosphorylcholine glyceride transferase, and malate synthase in endoplasmic reticulum (ER) isolated from endosperm on linear sucrose gradients. GA3 treatment also resulted in increased RNA associated with ER. Malate synthase and catalase in crude homogenates were enhanced by 1 to 100 μM GA3 concentrations. Isocitrate lyase, citrate synthase, malate synthase, catalase, and glycolate oxidase in isolated glyoxysomes were enhanced by 60, 20, 18, 40, and 28%, respectively, over controls. Treatment with abscisic acid led to decreased levels of glyoxysomal enzymes and reduced glyoxysomal protein. The effect of GA3 and abscisic acid on the specific activities of glyoxysomes of different densities suggests that GA3 influences enzyme levels and glyoxysome assembly.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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