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. 2024 Mar 7;12(3):e008652. doi: 10.1136/jitc-2023-008652

Figure 1.

Figure 1

TAA-specific memory T cell subsets in the lung. BALB/c mice were vaccinated either intranasally (i.n.) or intramuscularly (i.m.) with Ad4T1 (107 IU) + AdIL1β or Adempty (2.5×106 IU) as adjuvant component (A) and sacrificed 35 days later for T cell analysis. CD44 was used as a marker for antigen-experienced T cells with the addition of an intravascular CD45 staining to discriminate circulating (iv+) and tissue-resident (iv–) memory cells. The bars represent the mean of each group±SEM (B). The phenotypic analysis of effector T cells (TEFF; CD127−KLRG1+), effector memory T cells (TEM; CD127+KLRG1+), central memory T cells (TCM; CD127+KLRG1−CD69−CD103−) and TRM cells (CD127+/−KLRG1−CD103+CD69+; C) with AH1-specific TRM within the iv− population (D). PD1 and TIM-3 expression within different T cell phenotypes. TEFF, TEM and TCM analyzed in Ad4T1+AdIL1β i.m. and TRM populations analyzed in Ad4T1+AdIL1β i.n. group (E). The gating strategies are shown in online supplemental figure 1. Bars display group means overlaid with individual data points (C–E); all groups n=6 (total 24 animals). Significances were determined using Kruskal-Wallis tests followed by Dunn‘s multiple comparisons test (B–E). P values indicate significant differences (*p<0.05; **p<0.005; ***p<0.0005; ****p<0.0001; only statistically significant comparisons shown). CMV, cytomegalovirus promotor; i.m., intramuscular; i.n., intranasal; iv, intravenous, PD1, programmed cell death protein 1; T cell immunoglobulin and mucin-domain containing-3, TIM-3; TRM, tissue-resident memory T cells.