Figure 3.

Vaccine efficacy in a prophylactic setting. BALB/c mice were vaccinated with Ad4T1 (10⁷ IU) + AdIL1β (2.5×10⁶ IU) i.n. or i.m. or, alternatively, with Adempty (10⁷ IU) + AdIL1β (2.5×10⁶ IU) i.n. and challenged with 4T1-luc cells intravenously 35 days later. Survival was monitored for a period of 8 weeks (A). Bioimaging was performed on day 14 after tumor induction in an IVIS imaging system after the injection of mice with D-luciferin (B). Signal intensities are shown for individual animals in each group (C). The background signal is shown as a dashed line, indicating the detection limit (DL). Survival analysis over an 8-week period (D). The individual time until animals reach humane endpoints is shown together with group means. The experiment was terminated at day 56 and animals surviving to that time point were set to a survival of 56 days (E). Bars display group means overlaid with individual data points, all groups n=6 (total 24 animals). Significances were determined using Kruskal-Wallis tests followed by Dunn‘s multiple comparisons test (C, E). Significances in survival were determined using Gehan-Breslow-Wilcoxon-test (D). P values indicate significant differences (*p<0.05; only statistically significant comparisons shown). i.m., intramuscular; i.n., intranasal; IU, infectious units; iv, intravenous.