Figure 7.

Vaccine efficacy after therapeutic vaccination in combination with RT. BALB/c mice were challenged with 4T1-luc cells intravenously and received whole thorax radiation with a single dose of 5 Gy on day 5. One day later, they were vaccinated i.n. with Ad4T1 (10⁷ IU) + AdIL1β (2.5×10⁶ IU) (A). Survival analysis over an 8-week period (B). The individual time until animals reach humane endpoints is shown together with group means. The experiment was terminated at day 56 and animals surviving to that time point were set to a survival of 56 days. Bars display group means overlaid with individual data points with significances determined using Kruskal-Wallis tests followed by Dunn‘s multiple comparisons test (C). Weekly in vivo bioimaging in an IVIS imaging system after the injection of mice with D-luciferin (D). Significances in survival were determined using Gehan-Breslow-Wilcoxon test (B). n=7 for Ad4T1+AdIL1β i.n., other groups n=14 (total 49 animals). P values indicate significant differences (*p<0.05; **p<0.005; ***p<0.0005). Gy, Gray; i.n., intranasal; IU, infectious units; i.v., intravenous; RT, radiotherapy.