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. 2024 Mar 8;20:89. doi: 10.1186/s12917-024-03935-x

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 13 — The activity of serum complement components in the groups vaccinated with S. iniae and V. harveyi in Asian seabass. The effect of time in all groups was not significant (P > 0.05). Interaction of time × treatment: P = 0.6217. Values are Mean ± SD (n = 9 fish per treatment). Statistical significance was measured using Tukey’s test and two-way ANOVA to show the difference groups over time. CTRL: control, PBSI: Phosphate-buffered saline injection, SI: S. iniae injection, VI: V. harveyi injection, SVI: S. iniae and V. harveyi injection, SIM: S. iniae immersion, VIM: V. harveyi immersion, SVIM: S. iniae and V. harveyi immersion