Table 3.
Mean and standard deviation for the Ca Root Mean Square Deviation (RMSD) between each copy of the molecule in Structure 1 and Structure 2. The number of noncrystallographic symmetry-related copies in each structure is indicated in brackets. CPE: Clostridium perfringens enterotoxin. DN37CPE: CPE with the N-terminal 37 residues deleted. DN37CPE-D48A: DN37CPE with Asp48 mutated to Ala. CPE-CLD2: DN37CPE complexed with a peptide derived from mouse Claudin-2 extracellular loop II.
| (a) Monomers | ||||
|---|---|---|---|---|
| Å | Full-length CPE (3) | DN37CPE (6) | DN37CPE-D48A (6) | CPE-CLD2 (15) |
| CPE-CLD2 (15) |
0.81 ± 0.21 | 0.72 ± 0.1 | 0.72 ± 0.09 | 0.26 ± 0.1 |
| DN37CPE-D48A (6) | 0.68 ± 0.1 | 0.34 ± 0.11 | 0.38 ± 0.07 | |
| DN37CPE (6) | 0.70 ± 0.09 | 0.36 ± 0.09 | ||
| Full-length CPE (3) | 0.56 ± 0.13 | |||
| (b) Trimers | ||||
| Å | Full-length CPE (1) | DN37CPE (2) | DN37CPE- D48A (2) | CPE-CLD2 (5) |
| CPE-CLD2 (5) |
1.04 ± 0.03 | 1.1 ± 0.05 | 1.2 ± 0.09 | 0.35 ±0.1 |
| DN37CPE-D48A (2) | 0.99 ± 0.05 | 0.15 ± 0.02 | 0.61 (no SD) | |
| DN37CPE (2) | 0.99 ± 0.003 | 0.57 (no SD) | ||
| Full-length CPE (1) | N/A | |||