Figure 2.

The + 51 enhancer activates TAL1 promoter 1 in the erythroid lineage by recruiting the hSET1 HMT complex. (a) ChIP-seq analyses of H3K4me1, H3K4me3 and TAL1 binding at the TAL1 locus in CD34⁺ HSCs. (b) ChIP-seq analyses of TAL1 binding, hSET1 recruitment, H3K4me1 and H3K4me3 at the TAL1 locus in CD36⁺ erythroid precursors. (c) ChIP analysis of hSET1 recruitment at the TAL1 locus in K562 cells (top) and T-ALL Jurkat (bottom) cells. Data are shown as mean ± s.d. *P < 0.05; **P < 0.01. (d) K562 and several T-ALL cells were transfected with a control pGL3-SV40 luciferase reporter, a + 51 enhancer-driven pGL3-SV40 luciferase reporter, or a + 51 enhancer-driven pGL3-SV40 luciferase reporter containing a + 53 CTCF site inserted between the + 51 enhancer and the SV40 promoter. A CMV-driven renilla luciferase plasmid was used as a transfection control. Transfected cells were cultured for 48 h and lysed for the measurement of luciferase activity (RLU). Data are shown as mean ± s.d. *P < 0.05.