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. Author manuscript; available in PMC: 2024 Mar 8.
Published in final edited form as: Leukemia. 2013 May 23;28(2):349–361. doi: 10.1038/leu.2013.158

Figure 7.

Figure 7.

KD of c-Maf leads to disruption of the interchromosomal interaction and decrease in TAL1 transcription in T-ALL cells. (a) ChIP analysis of T-cell-specific transcription factor c-Maf binding in the CD2BP2 and TAL1 loci in Jurkat cells. Data are shown as mean ± s.d. of three independent experiments. *P < 0.05; **P < 0.01. (b, c) Western blot (b) and RT-qPCR (c) analyses of TAL1 expression in the vector control and two c-Maf KD clones. (d) 3C analysis of interchromosomal interaction between TAL1 promoter 1 and the TIL16 element comparing the vector control and c-Maf KD Jurkat clones. A total of three independent 3C experiments were quantitated by densitometry. (e, f) ChIP analyses of H3K4me3 levels (e) as well as TAF3 recruitment (f) at the + 51 enhancer and TAL1 promoter 1 upon c-Maf KD. Data are shown as mean ± s.d. of three independent experiments. *P < 0.05; **P < 0.01.