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. 1998 Nov;18(11):6365–6373. doi: 10.1128/mcb.18.11.6365

FIG. 5.

FIG. 5

Kin28 phosphorylation and activity in wild-type and cak1Δ cells carrying Cak1-independent Cdc28 mutants. Myc-tagged Kin28 mobility on Western blots (top) and CTD kinase activity in immunoprecipitates (bottom) were measured in wild-type (wt) cells (lane 1), cak1-23 cells at room temperature (lane 2), and cells carrying Cak1-independent Cdc28-4324 (lanes 3 and 4) or Cdc28-5331 (lanes 5 and 6), in the presence (lanes 3 and 5) or absence (lanes 4 and 6) of the CAK1 gene. Lane C is a control sample from cells lacking epitope-tagged Kin28.