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Cak1-dependent activation of Kin28 in insect cells. Sf9 cells were coinfected with recombinant baculoviruses encoding the indicated combinations of Kin28, Ccl1, Tfb3, and Cak1. Kin28 was immunoprecipitated from cell lysates 2 days after infection and analyzed by Western blotting (top) and CTD kinase activity (bottom) assays. All the cells in this experiment were also coinfected with a virus encoding yeast Cdc37 (17), which increases Cak1 expression in insect cells.
