TABLE 2.
Phenotypes of cak1 mutants
| Strain | % of total populationa that was:
|
|||
|---|---|---|---|---|
| Unbuddedb | Small buddedc | Large buddedd | Elongated budding | |
| Log phase at 25°C | ||||
| Wild type | 49 | 23 | 28 | 0 |
| cak1-23 | 47 | 30 | 22 | <1 |
| cak1-34 | 40 | 36 | 23 | <1 |
| cak1-95 | 41 | 35 | 23 | <1 |
| cdc28-4 | 43 | 30 | 27 | 0 |
| kin28-3 | 51 | 22 | 26 | <1 |
| cdc28-4 kin28-3 | 42 | 34 | 23 | <1 |
| 3 h at 37°C | ||||
| Wild type | 48 | 26 | 25 | <1 |
| cak1-23 | 36 | 18 | 4 | 41 |
| cak1-34 | 43 | 13 | 3 | 40 |
| cak1-95 | 70 | 3 | 5 | 14 |
| cdc28-4 | 82 | <1 | 18 | <1 |
| kin28-3 | 46 | 26 | 26 | <1 |
| cdc28-4 kin28-3 | 62 | 7 | 2 | 19 |
| Release from G1 at 37°Ce | ||||
| cak1-23 | 52 | 7 | <1 | 42 |
| cak1-34 | 33 | <1 | <1 | 67 |
| cak1-95 | 65 | <1 | <1 | 34 |
| cdc28-4 | 97 | 3 | <1 | 0 |
| kin28-3 | 15 | 8 | 73 | 0 |
a
n > 300 cells.
b
Includes cells with mating projections.
c
Bud size less than 50% of the size of the mother cell.
d
Bud size more than 50% of the size of the mother cell.
e
Mid-log-phase cells were treated with alpha-factor at 25°C until >90% of the cells were arrested in G1, shifted to 37°C for 30 min, and then released from the arrest at 37°C for 3 h.