FIG. 2.

Genetic interaction between LOS1 and GCD11. Shuffle strain YKH53 (los1::HIS3 gcd11::HIS3) containing pRS316-GCD11 was transformed with various GCD11 alleles on a LEU2 plasmid and then streaked on 5-FOA-containing medium, on which cells with the URA3 plasmid, which contains the GCD11 wild-type gene, were selected against. The GCD11 point mutations G397A and R510H, combined with a los1::HIS3 disruption, led to synthetic lethality (left), which was overcome by cotransformation with a LOS1 wild-type gene on a TRP1 plasmid (right).