TABLE 2.

Quantification of the two-hybrid interaction between Los1p and Nup2p, Gcd11p, Gsp1p, or Gsp2p with LOS1 bait^a

Activity with LOS1 bait and prey plasmid:
NUP2N (42–177)	GCD11C (368–527)	GSP1	GSP2	SNF4	No insert
23	1.9	0.70	0.73	0.01	0.03

^aβ-Galactosidase activities of yeast strain Y190(pAS2-LOS1), which contained the indicated prey plasmids (pACTII derived), were measured by the ONPG liquid assay (see Materials and Methods). For each combination, the average of data for at least three independent transformants was calculated. Prey plasmids pACT-SNF4 and pACTII without inserts served as negative controls. Activity, calculated by the equation 1,000 × (OD₄₂₀/OD₆₀₀ × V × t), is expressed in milliliters per minute.