Figure 2.

Wide-SIM/MS² and wSIM-City identification of known AA-I and novel AA DNA adducts in kidneys of untreated and 13-carcinogen-mixture treated rats 24 h after dosing. The precursor ions are depicted with black sticks, and the aglycones are depicted with red sticks. The arrows point towards the internal standards [¹⁵N₅]-dA-N⁶-AL-I and [¹⁵N₅]-dG-N²-AL-I. The top two rows represent dA-N⁶-AL-I and dG-N²-AL-I and their putative isomers, and the bottom two rows show [¹⁵N₅]-dA-N⁶-AL-I and [¹⁵N₅]-dG-N²-AL-I. The EIC data are from wSIM-City automated identification of the precursor and aglycone m/z peak intensities for each scan that showed a detected neutral loss of dR within a 5 ppm mass tolerance in the scan immediately following the precursor ion scan.