Figure 5. HSD does not alter AgRP neuron responses to lipids or peptides.

(A and B) Calcium signal in AgRP neurons from fasted NCD (A) and HSD (B) mice during intragastric intralipid delivery at baseline, after 4 weeks of chow or HSD (4 weeks), and after an additional 4 weeks of chow (8 weeks) as indicated. n = 4–8 mice per group.

(C and D) Average ΔF/F in NCD (C) and HSD (D) mice from (A) and (B) for 1 min surrounding the end of the infusion (C: one-way ANOVA, p = 0.7015; D: one-way ANOVA, p = 0.1705).

(E–G) Heatmaps portraying AgRP neuron fluorescence changes in each HSD mouse from (B) and (D) at baseline (E), after 4 weeks of HSD (F), and after 4 more weeks of regular chow (G).

(H and I) Calcium signal in AgRP neurons from fasted NCD (H) and HSD (I) mice during intragastric peptide delivery at baseline, after 4 weeks of chow or HSD (4 weeks), and after an additional 4 weeks of chow (8 weeks) as indicated. n = 5 mice per group.

(J and K) Average ΔF/F in NCD (J) and HSD (K) mice from (H) and (I) for 1 min surrounding the end of the infusion (J: one-way ANOVA, p = 0.2826; K: one-way ANOVA, p = 0.7199).

(L–N) Heatmaps portraying AgRP neuron fluorescence changes in each HSD mouse from (I) and (K) at baseline (L), after 4 weeks of HSD (M), and after 4 more weeks of regular chow (N).

(O and P) Calcium signal in AgRP neurons from fasted NCD (O) and HSD (P) mice during intragastric water delivery at baseline, after 4 weeks of chow or HSD (4 weeks), and after an additional 4 weeks of chow (8 weeks) as indicated. n = 4–5 mice per group.

(Q and R) Average ΔF/F in NCD (Q) and HSD (R) mice from (O) and (P) for 1 min surrounding the end of the infusion (Q: one-way ANOVA, p = 0.3332; R: one-way ANOVA, p = 0.5615).

(A, B, H, I, O, and P) Isosbestic traces for all recordings are shown in gray. (E–G and L–N) Each heatmap line represents an individual animal. (C, D, J, K, Q, and R) Lines represent individual mice. Error bars indicate mean ± SEM.

See also Figure S5.