KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
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| Rabbit polyclonal anti-GFP | Invitrogen | REF: A11122; RRID: AB_221569 |
| Donkey polyclonal anti-rabbit | Jackson ImmunoResearch | Code: 711-545-152; RRID: AB_2313584 |
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| Bacterial and virus strains | ||
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| pAAV.CAG.Flex.GCaMP6s.WPRE.SV40 | (Chen et al.)53 | Addgene; Cat#: 100842-AAV9 |
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| Chemicals, peptides, and recombinant proteins | ||
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| Acarbose, 95% | Acros Organics | Cat#: AC459080010 |
| Cholecystokinin Octapeptide (sulfated) | Bachem | PN#: 4033010 |
| Collagen Peptides (Unflavored) | Sports Research | N/A |
| D-(−)-Fructose | Tokyo Chemical Industry | PN#: F0060 |
| D-(+)-Glucose | Sigma-Aldrich | Cat#: G8270 |
| Hershey’s Kisses Milk Chocolate Candy | The Hershey Company | N/A |
| Intralipid 20% | Fresnius Kabi | NDC: 0338-0519-09 |
| Peptide YY (3-36) | Tocris Bioscience | Cat#: 1618 |
| Sotagliflozin (LX4211) | Selleck Chemical | Cat#: S8103 |
| Sucrose, 99% | Thermo Fisher Scientific | Cat#: A15583.0C |
| Teklad LM-485 Mouse/Rat Sterilizable Diet | Envigo | Product code 7012 |
| Normal Donkey Serum | Jackson ImmunoResearch | Code: 017-000-121 |
| Triton X-100 | EMD Millipore | Cat#: TX1568-1 |
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| Critical commercial assays | ||
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| iScript cDNA Synthesis Kit | Bio-Rad | Cat#: 1708891 |
| iTaq Universal SYBR Green Supermix | Bio-Rad | Cat#: 1725120 |
| Mouse Leptin ELISA Kit | Crystal Chem Immunoassays | Cat#: 90030 |
| Mouse Insulin ELISA Kit | Crystal Chem Immunoassays | Cat#: 90080 |
| RNeasy Mini Kit | Qiagen | Cat#: 74104 |
| VetScan Mammalian Liver Profile | Abaxis | Cat#: 500-0040-12 |
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| Experimental models: Organisms/strains | ||
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| C57BL6/J | The Jackson Laboratory | Strain #: 000664 |
| Agrp tm1(cre)Lowl /J | The Jackson Laboratory | Strain #: 012899 |
| B6; 129S-Gt(ROSA) 26Sortm32(CAG-COP4’H124R/EYFP)Hze/j | The Jackson Laboratory | Strain #: 012569 |
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| Oligonucleotides | ||
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| Primers for qPCR, see Table S2 | This paper | N/A |
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| Software and algorithms | ||
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| Fiber photometry analysis scripts | This paper | https://doi.org/10.5281/zenodo.10403577 |
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| Other | ||
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| Fiber photometry implant | Doric Lenses | MFC_400/430-0.48_6.3mm_MF2.5_FLT |
| Bronze mating sleeve | Doric Lenses | SLEEVE_BR_2.5 |
| Syringe Pump | Harvard Apparatus | Cat#: 70-2000 |
| Polyurethane tubing for nutrient infusions | Instech Laboratories | VAHBPU-T25 |
| Adaptors for nutrient infusions | Instech Laboratories | PNP3MC/25; LS25 |
| Intragastric catheter | Instech Laboratories | C30PU-MGA1909 |
| Felt button | Instech Laboratories | VABM1B/22 |
| Magnetic cap | Instech Laboratories | VABM1C |
| Photometry processors | Tucker-Davis Technologies | RZ5P; RZ10X |
| LEDs | Thorlabs | M405FP1; M470F3 |
| LED Driver | Thorlabs | DC4100 |
| Photometry patch cords | Doric Lenses | MFP_400/430/1100-0.57_2m_FCM-MF2.5_LAF |
| Optogenetics patch cords | Prizmatix | 500um POF N.A. 0.63 L = 75cm |
| LED source + programmable TTL pulse generator for optogenetics | Prizmatix | PN#: D-OG-LED-B/B |
| Optogenetics implant | Doric Lenses | MFC_200/245_0.37_6.1mm_ZF1.25_FLT |
| Zirconia mating sleeve | Doric Lenses | F210-3001 |