Table 4.
Comparison of different antibody clones in ABC value determination
| Conjugate/Mfr. | ABC value (F/P adjusted) |
|---|---|
| CD19 BC clone J3-119: F/P 1.19 | |
| Mean | 9,941 (8,354) |
| S.D. | 95 |
| CV | 0.0096 |
| CD19 BDIS clone SJ25C1: F/P 1.018 | |
| Mean | 6,789 (6,669) |
| S.D. | 185 |
| CV | 0.0272 |
| CD20 BC clone B9E9: F/P 1.0 | |
| Mean | 12,398 (12,398) |
| S.D. | 430 |
| CV | 0.0346 |
| CD20 BDIS clone L27: F/P 0.897 | |
| Mean | 19,480 (21,717) |
| S.D. | 397 |
| CV | 0.0204 |
| CD22 BC clone SJ10.1H11: F/P 0.8 | |
| Mean | 5,066 (6,333) |
| S.D. | 69 |
| CV | 0.0136 |
| CD22 BDIS clone S-HCL-1: F/P 1.03 | |
| Mean | 9,710 (9,427) |
| S.D. | 14 |
| CV | 0.0014 |
| CD4 BC clone 13B8.2: F/P 1.2 | |
| Mean | 33,715 (28,096) |
| S.D. | 340 |
| CV | 0.0101 |
| CD4 BDIS clone SK3: F/P 1.094 | |
| Mean | 29,721 (27,167) |
| S.D. | 160 |
| CV | 0.0054 |
Mean, Standard Deviation (SD), and Coefficient of Variation (CV) from triplicate Cytotrol cells stained with different antibody clones from BC-Beckman Coulter and BDIS-BD Bioscences and run on a BDIS FACSCanto.