Table 6.
Comparison of CD4 Raw GeoMFI and ABC Value Variability Across Instruments
| Flow Cytometer | CD4 Raw GeoMFI | CD4 ABC value |
|---|---|---|
| CytoFLEX LX | ||
| Mean | 75294 | 24,472 |
| SD | 1204 | 385 |
| CV | 0.0160 | 0.0157 |
| Attune NxT | ||
| Mean | 47602 | 23,129 |
| SD | 363 | 181 |
| CV | 0.0076 | 0.0078 |
| CANTO | ||
| Mean | 18717 | 25,166 |
| SD | 29 | 39 |
| CV | 0.0015 | 0.0015 |
| Lyric | ||
| Mean | 27134 | 26,089 |
| SD | 509 | 488 |
| CV | 0.0188 | 0.0187 |
Mean, Standard Deviation (SD), and Coefficient of Variation (CV) from triplicate Cytotrol cells stained with CD4PE, clone SK3 and CD45 v500, clone H130 (BDIS). No F/P ratio adjustment was made to the ABC values.