. Author manuscript; available in PMC: 2025 Jan 12.

Published in final edited form as: Cytometry B Clin Cytom. 2024 Jan 12;106(1):25–34. doi: 10.1002/cyto.b.22155

Table 7.

Comparison of CD20 ABC variability using the Off-the-Shelf (OTS) and Unimolar (U) CD20 PE conjuagtes Across Instruments

Conjugate/Mfr.	Raw GeoMFI	ABC value^*	ABC value with F/P corrected	(ABC_CD20 / ABC_CD4) ×40,000 (CD4 ABC from Table 8 as a reference marker)^*
CytoFLEX LX
CD20 OTS, [F/P=0.946]: Mean (SD) [CV]	34,123 (1014) [0.0297]	11,247 (328) [0.292]	11,889 (347) [0.0292]	18,384 (1074) [0.0584]
CD20 U, [F/P=1.094): Mean (SD) [CV]	50,782 (692) [0.0136]	16,843 (222) [0.0132]	17,550 (235) [0.0134]	27,530 (363) [0.0132]
Attune NxT
CD20 OTS: Mean (SD) [CV]	17,028 (3649) [0.2143]	8,063 (1767) [0.2191]	8,524 (1869) [0.2193]	13,945 (3058) [0.2193]
CD20 U, [F/P=1.094): Mean (SD) [CV]	31,961 (2129) [0.0666]	15,373 (1049) [0.682]	16,234 (1108) [0.0682]	26587 (1815) [0.0683]
Canto II
CD20 OTS, [F/P=0.946]: Mean (SD) [CV]	6,158 (1711) [0.2778]	8,358 (2301) [0.2753]	8,835 (2433) [2754]	13,285 (3658) [0.2753]
CD20 U, [F/P=1.094): Mean (SD) [CV]	12,402 (803) [0.0647]	16,735 (1075) [0.0642]	17,672 (1135) [0.0642]	26,600 (1709) [0.0642]
Lyric
CD20 OTS, [F/P=0.946]: Mean (SD) [CV]	11,916 (224) [0.0188]	11,469 (216) [0.0188]	12,124 (224) [0.0185]	17,584 (343) [0.0195]
CD20 U, [F/P=1.094): Mean (SD) [CV]	17,964 (1178) [0.0656]	17,282 (1132) [0.0655]	18,249 (1196) [0.0655]	26,497 (1799) [0.0679]

Indicates no F/P ratio adjustment. Mean, Standard Deviation (SD), and Coefficient of Variation [CV] from triplicate Cytotrol cells stained with off-the-shelf CD20 PE, clone L27 and CD45 v500, clone H130 or a second cocktail with unimolar CD20 PE, clone L27 and CD45 v500, clone H130, both from BD Bioscences.