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. Author manuscript; available in PMC: 2025 Jan 12.
Published in final edited form as: Cytometry B Clin Cytom. 2024 Jan 12;106(1):25–34. doi: 10.1002/cyto.b.22155

Table 8.

Comparison of CD20 ABC variability using three different quantification approaches across instruments

CD20 OTS ABC (ABCCD20OTS/ABCCD4OTS) ×40,000 (GeoMFICD20OTS/GeoMFICD4OTS) × 40,000 CD20 1:1 ABC (ABCCD201:1/ABCCD4OTS) ×40,000 (GeoMFICD201:1/GeoMFICD4OTS) × 40,000
CytoFlex LX 11,247 18,384 18,128 16,843 27,530 26,978
Attune NxT 8,063 13,945 14,308 15,373 26,587 26,856
CANTO 8,358 13,285 13,160 16,735 26,600 26,505
Lyric 11,469 17,584 17,872 17,282 27,469 26,482
Mean 9784 15,800 15,867 16,558 26,956 26,705
SD 1824 2558 2509 825 436 250
CV 0.186 0.162 0.158 0.050 0.016 0.009

CD20 OTS: Non-unimolar off the shelf CD20. CD20 1:1: Unimolar CD20. ABC values are from Table 7 and are not corrected by F/P ratio.