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. 1978 Dec;62(6):930–932. doi: 10.1104/pp.62.6.930

Thymidine Phosphorylation in Wheat

Analysis of Phosphate Transfer from ATP to Thymidine 1

Jason Hofman 1, Otto J Schwarz 1
PMCID: PMC1092258  PMID: 16660642

Abstract

Extracts of wheat (Triticum vulgare Vill. [Triticum aestivum L.] var. Lemhi) seedlings contain thymidine-phosphorylating activity with ATP, ADP, or AMP and nucleotide hydrolase activity (ATP → → AMP). The synthesis of [32P]dTMP exclusively from [α-32P]ATP with none detectable from [γ-32P]ATP demonstrates the absence of thymidine kinase and the presence of nucleoside phosphotransferase as the only observable thymidine-phosphorylating enzyme.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barton R. A., Schulman A., Jacobson E. L., Jacobson M. K. Chromatographic separation of pyridine and adenine nucleotides on thin layers of poly(ethyleneimine) cellulose. J Chromatogr. 1977 Jan 11;130:145–150. doi: 10.1016/s0021-9673(00)89790-3. [DOI] [PubMed] [Google Scholar]
  2. Brunngraber E. F., Chargaff E. A nucleotide phosphotransferase from Escherichia coli. Purification and properties. Biochemistry. 1973 Jul 31;12(16):3005–3012. doi: 10.1021/bi00740a009. [DOI] [PubMed] [Google Scholar]
  3. Deng Q. I., Ives D. H. Modes of nucleoside phosphorylation in plants: studies on the apparent thymidine kinase and true uridine kinase of seedlings. Biochim Biophys Acta. 1972 Aug 25;277(2):235–244. [PubMed] [Google Scholar]
  4. Glynn I. M., Chappell J. B. A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity. Biochem J. 1964 Jan;90(1):147–149. doi: 10.1042/bj0900147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  6. OKAZAKI R., KORNBERG A. DEOXYTHYMIDINE KINASE OF ESCHERICHIA COLI. I. PURIFICATION AND SOME PROPERTIES OF THE ENZYME. J Biol Chem. 1964 Jan;239:269–274. [PubMed] [Google Scholar]
  7. Randerath K., Randerath E. Ion-exchange thin-layer chromatography. XV. Preparation, properties and applications of paper-like PEI-cellulose sheets. J Chromatogr. 1966 Apr;22(1):110–117. doi: 10.1016/s0021-9673(01)97076-1. [DOI] [PubMed] [Google Scholar]
  8. Schwarz O. J., Haber A. H. Thymidine-phosphorylating Activity in gamma-Plantlets: Separation of Onset of Activity from Deoxyribonucleic Acid Synthesis. Plant Physiol. 1973 May;51(5):984–986. doi: 10.1104/pp.51.5.984. [DOI] [PMC free article] [PubMed] [Google Scholar]

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