Figure 1.

Overview of study design and phenotypes. A, Overall discovery strategy. Wild-type (WT) and hyperglycemic type 1 diabetic Akita mice were put on a high-fat, high-carbohydrate Western diet for 4 weeks, followed by SGLT2i (sodium-glucose cotransporter 2 inhibitor), dapagliflozin (10 mg/kg of Western diet), or vehicle (repelleted Western diet) for 1 week. B, Organs and body fluids were snap-frozen and subjected to multi-omics analysis using mass spectrometry. SGLT2 interactome was determined from kidney. C, Main findings. D, Overview of functional follow-up studies and key findings. GDF15 indicates growth/differentiation factor 15; RBC, red blood cell; and WAT, white adipose tissue.