FIG. 3.

Importance of the S and A regions of IL-2Rβ for Akt IL-2-induced activation. (A) 32D cells expressing either wild-type (βWT) or mutant forms of human IL-2Rβ were rested overnight and then stimulated with IL-2 for 15 min. In vitro kinase (IVK) assays were performed on Akt immunocomplexes, using histone H2B peptide as an exogenous substrate. (B) Loading control for the in vitro kinase assay. Immunoprecipitation (IP) with anti-Akt was followed by Western blotting with anti-Akt antibody. (C) Receptor expression controls are also shown for each cell line.