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The JH1 and JH2 regions of Jak1 are required for interaction with p85. Jak2 and Jak3 do not interact with p85. (A) 293 T+ cells were transfected with p85 and with the indicated forms of FLAG-tagged Jak1 C-terminal deletions or FLAG-tagged Jak2 or Jak3. Immunoprecipitations (IP) with anti-p85 antibody were followed by Western blotting with anti-FLAG M2 MAb. WT, wild type. (B) Expression control for the FLAG-tagged forms of Jak1, Jak2, and Jak3. (C) Expression controls for p85.
