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. 2005 May 10;33(8):2640–2649. doi: 10.1093/nar/gki554

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© The Author 2005. Published by Oxford University Press. All rights reserved

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Specific binding of [³²P]pRNA I-i′ to connector demonstrated by UV crosslinking assay. Lanes A–D, 10% SDS–PAGE autoradiographed pictures; lanes a–d, the same gel stained by Coomassie blue. Lane A, pRNA crosslinked to connector and then treated by RNase A; lane B, procapsid crosslinked to pRNA and then treated by RNase A; lane C, procapsid–pRNA complex without crosslinking, and then treated by RNase A; lane D, pRNA treated by RNase A. Lanes a, b, c and d correspond to A, B, C and D, respectively.