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. 2005 May 10;33(8):2640–2649. doi: 10.1093/nar/gki554

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© The Author 2005. Published by Oxford University Press. All rights reserved

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A total of 10% SDS–PAGE to show the connector protein gp10 and procapsid treated before and after V8 cleavage. Lane 1, procapsid alone; lane 2, procapsid cleaved by proteaseV8; lane 3, purified connector protein gp10 alone; lane 4, connector cleaved by V8; lane 5, connector–pRNA complex cleaved by V8. As noted in the text, in the procapsid, the C-terminus of gp10 is located at the wider end of the connector that is buried within the procapsid, while the N-terminus is located at the narrow end of the connector that is exposed to the solvent. Treatment of connector or procapsid with V8 resulted in different sizes of gp10, since V8 can cleave both the N- and C-terminus of gp10 of the free connector, but only the N-terminus of the gp10 that is buried within the procapsid. Gp8 is the capsid protein, while gp8.5 is the fiber protein of the procapsid.