Key resources table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit Anti-SLC25A39 | Proteintech | Cat#14963-1-AP |
| Rabbit Anti-VDAC | Cell Signaling Technology | Cat#4661T |
| Mouse Anti-GAPDH | Invitrogen | Cat#39-8600 |
| Mouse Anti-SLC25A40 | Abcam | Cat#ab69075 |
| Rabbit Anti-AFG3L2 | Proteintech | Cat#14631-1-AP |
| Mouse Anti-FLAG | GenScript | Cat#A00187 |
| Mouse Anti-NDUFA9 | Invitrogen | Cat#459100 |
| Rabbit Anti-HSCB | Proteintech | Cat#15132-1-AP |
| Rabbit Anti-Synaptophysin 1 | Synaptic Systems | Cat#101002 |
| Rabbit Anti-IRP2 | Cell Signaling Technology | Cat#37135S |
| Rabbit Anti-SLC25A37(Mitoferrin1) | Proteintech | Cat#26469-1-AP |
| Rabbit Anti-GLRX5 | Sigma | Cat#HPA042465 |
| Rabbit-Anti-ABCB7 | Proteintech | Cat#11158-1-AP |
| Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP | Invitrogen | Cat#PI31460 |
| Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP | Invitrogen | Cat#PI31430 |
| Chemicals, peptides, and recombinant proteins | ||
| L-Butathione Sulfoximine | Sigma | Cat#B2515 |
| Cycloheximide | Sigma | Cat#C4859 |
| Deferoxamine mesylate salt | Sigma | Cat#D9533 |
| GSH-[glycine-13C2, 15N] | Sigma | Cat#683620 |
| EZ-link Iodoacetyl-PEG2-Biotin | Thermo Scientific | Cat#21334 |
| DYKDDDDK Peptide | GenScript | Cat#RP10586 |
| Hygromycin B | Roche | Cat#10843555001 |
| Puromycin Dihydrochloride | Gibco | Cat#A1113803 |
| Blasticidin S hydrochloride | Alfa Aesar | Cat#J61883.FPL |
| Digitonin (5%) | Invitrogen | Cat#BN2006 |
| Doxycycline | Sigma | Cat#D9891 |
| Critical commercial assays | ||
| Pierce BCA protein assay kit | Thermo Scientific | Cat#23225 |
| Silver Stain Plus Kit | Bio-Rad | Cat#1610449 |
| Superscript™ III First-Strand Synthesis SuperMix for qRT-PCR | Invitrogen | Cat#11752050 |
| Lipofectamine 3000 Transfection kit | Invitrogen | Cat#L3000008 |
| RNeasy Mini kit | Qiagen | Cat#74104 |
| Universal Mycoplasma Detection kit | ATCC | Cat#30-1012K |
| Deposited data | ||
| The raw data for the proteomics have been deposited with the ProteomeXchange Consortium via the PRIDE partner repository. | This paper | PXD047327 |
| Experimental models: Cell lines | ||
| K562 cells | ATCC | CCL-243 |
| HeLa | Pietro De Camilli lab | ATCC-CCL-2 |
| HEK293T | ATCC | CRL-3216 |
| Human iPSC KOLF 2.1J line | Jackson Laboratories | JIPSC1000 |
| Oligonucleotides | ||
| spCas9 sgRNA guide sequences to generate the CRISPR KO cells | This paper | Table S2 |
| Recombinant DNA | ||
| lentiCRISPR v2 vector | Addgene | Cat#52961 |
| lentiCRISPR v2 - hygro | Broad institute | pXPR_BRD051 |
| lentiCRISPR v2 - Blast | Addgene | Cat#83480 |
| pMXs-3xHA-EGFP-OMP25 | Addgene | Cat#83356 |
| psPAX2 | Addgene | Cat#12260 |
| pMD2.G | Addgene | Cat#12259 |
| SLC25A39-FLAG (linker) - hygro resistance | This paper | N/A |
| SLC25A39FLAG (no linker) - hygro resistance | This paper | N/A |
| SLC25A394CA-FLAG - hygro resistance | This paper | N/A |
| SLC25A39C334S-FLAG - hygro resistance | This paper | N/A |
| SLC25A39A11L1-FLAG - hygro resistance | This paper | N/A |
| SLC25A39A40L1-FLAG - hygro resistance | This paper | N/A |
| SLC25A39C74A-FLAG - hygro resistance | This paper | N/A |
| SLC25A39C78A-FLAG - hygro resistance | This paper | N/A |
| SLC25A39C88A-FLAG - hygro resistance | This paper | N/A |
| SLC25A39C94A-FLAG - hygro resistance | This paper | N/A |
| AFG3L2-3xFLAG - hygro resistance | This paper | N/A |
| Software and algorithms | ||
| GraphPad Prism 10.1.1 | GraphPad Software | N/A |
| Xcalibur 4.1 | Thermo Scientific | N/A |
| StepOne Software version 2.1 | Applied Biosystems | N/A |
| FIJI (ImageJ2) | https://imagej.net/software/fiji/ | N/A |
| Proteome Discoverer version 2.5 | Thermo Scientific | N/A |
| Other | ||
| human SLC25A28 TaqMan probe | Thermo Fisher Scientific | Cat#4331182/Hs00945863_m1 |
| human SLC25A39 TaqMan probe | Thermo Fisher Scientific | Cat#4331182/Hs00924971_g1 |
| human ABCB7 TaqMan probe | Thermo Fisher Scientific | Cat#4331182/Hs00188776_m1 |
| human AFG3L2 TaqMan probe | Thermo Fisher Scientific | Cat#4351372/Hs01064997_m1 |