TABLE 1.
Yeast strains
| Strain | Genotype |
|---|---|
| DY150a | MATa |
| DY161a | MATa swi5::LEU2 |
| DY1133a | MATα ace2::TRP1 swi5::hisG |
| DY1143a | MATa ace2::HIS3 swi5::hisG |
| DY1921a | MATa pho2::LEU2 |
| DY1923a | MATa ace2::TRP1 pho2::LEU2 |
| DY1936a | MATa pho2::LEU2 swi5::hisG-URA3-hisG |
| DY1985a | MATa ace2::HIS3 pho2::LEU2 swi5::hisG |
| DY4174a | MATa ace2::LEU2 swi5::hisG |
| DY4684a | MATα ace2::HIS3 SWI5*(E482K)d |
| DY4686a | MATa ace2::TRP1 SWI5*(S483G) |
| DY4688a | MATa ace2::TRP1 SWI5*(R484S) |
| DY4690a | MATa ace2::HIS3 SWI5*(F485S) |
| DY4692a | MATa ace2::TRP1 SWI5*(V494A) |
| DY4695a | MATa ace2::TRP1 SWI5*(S497P) |
| DY4696a | MATa ace2::TRP1 SWI5*(Q498R) |
| DY4698a | MATa ace2::TRP1 SWI5*(S505P) |
| DY4852a | MATa ace2::TRP1 SWI5*(R484G) |
| DY4854a | MATa ace2::TRP1 SWI5(WTe) |
| DY1641b | MATa URA3::8-lexA-CYC1::lacZ |
| DY4678b | MATa ace2::LEU2 swi5::hisG URA3::HO(site B)-CYC1::lacZ |
| DY4680b | MATa ace2::hisG swi5::hisG URA3::CTS1(46)-CYC1::lacZ |
| DY2406c | MATa HO(a1) hmla HMRa pho2::LEU2 |
| DY4843c | MATa HO(a1) hmla HMRa swi5::URA3 |
| DY4905c | MATa HO(a1) hmla HMRa SWI5*(E482K) |
| DY4907c | MATa HO(a1) hmla HMRa SWI5*(S483G) |
| DY4909c | MATa HO(a1) hmla HMRa SWI5*(R484G) |
| DY4911c | MATa HO(a1) hmla HMRa SWI5*(R484S) |
| DY4913c | MATa HO(a1) hmla HMRa SWI5*(F485S) |
| DY4915c | MATa HO(a1) hmla HMRa SWI5*(V494A) |
| DY4917c | MATa HO(a1) hmla HMRa SWI5*(S497P) |
| DY4919c | MATa HO(a1) hmla HMRa SWI5*(Q498R) |
| DY4921c | MATa HO(a1) hmla HMRa SWI5*(S505P) |
| DY5031c | MATa HO(a1) hmla HMRa SWI5(WT) |
a
Strain that is isogenic in the W303 background (38) and has these additional markers: ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1.
b
W303 strain that has the indicated reporter integrated at the URA3 locus.
c
Strain that is isogenic in the K765 background (29) and has these additional markers: ade2-1 ade6 can1-100 his3-11,15 leu2-3,112 met- trp1-1 ura3-52.
d
For SWI5* mutants, encoded point mutations are shown in parentheses.
e
WT, wild type.