TABLE 2.
Plasmids
| Plasmid | Description |
|---|---|
| M3405 | swi5::URA3 disruption plasmid |
| M2667 | SWI5 in YEplac112 |
| M1853 | HO(site B)-lacZ reporter, YEp with URA3 marker |
| M1912 | CTS1(46)-lacZ reporter, YEp with URA3 marker |
| M3403 | HO(site B)-lacZ reporter, YIp version with URA3 marker |
| M3404 | CTS1(46)-lacZ reporter, YIp version with URA3 marker |
| M3202 | SWI5 with two new BamHI sites in open reading frame in pRS313 |
| M2024 | Expresses His-tagged Swi5 in E. coli |
| M2025 | Expresses His-tagged Pho2 in E. coli |
| M3113 | pET-16b:SWI5 (wild type) |
| M3635 | pET-16b:SWI5*(E482K) |
| M3636 | pET-16b:SWI5*(S483G) |
| M3637 | pET-16b:SWI5*(R484G) |
| M3638 | pET-16b:SWI5*(R484S) |
| M3639 | pET-16b:SWI5*(F485S) |
| M3640 | pET-16b:SWI5*(V494A) |
| M3641 | pET-16b:SWI5*(S497P) |
| M3642 | pET-16b:SWI5*(Q498R) |
| M3643 | pET-16b:SWI5*(S505P) |
| pRS313 | YCp vector with HIS3 marker |
| M3114 | SWI5(wild type) in pRS313 |
| M3562 | SWI5*(T490A) in pRS313 |
| M3563 | SWI5*(S492A) in pRS313 |
| M3564 | SWI5*(S505A) in pRS313 |
| M3565 | SWI5*(T490A,S492A) in pRS313 |
| M3566 | SWI5*(S492A,S505A) in pRS313 |
| M3567 | SWI5*(T490A,S505A) in pRS313 |
| M3568 | SWI5*(T490A,S492A,S505A) in pRS313 |
| M3631 | SWI5*(S505P) in pRS313 |
| M1921 | pLexA202+PL, expresses LexA on YEp vector with HIS3 marker |
| M3895 | LexA-Swi5(WT,471-513) in pLexA202+PL |
| M3896 | LexA-Swi5(E482K,471-513) in pLexA202+PL |
| M3897 | LexA-Swi5(S483G,471-513) in pLexA202+PL |
| M3899 | LexA-Swi5(R484S,471-513) in pLexA202+PL |
| M3900 | LexA-Swi5(F485S,471-513) in pLexA202+PL |
| M3901 | LexA-Swi5(S497P,471-513) in pLexA202+PL |
| M3902 | LexA-Swi5(Q498R,471-513) in pLexA202+PL |
| M3903 | LexA-Swi5(S505P,471-513) in pLexA202+PL |
| M3917 | LexA-Swi5(R484G,471-513) in pLexA202+PL |
| M3918 | LexA-Swi5(V494A,471-513) in pLexA202+PL |
| M3931 | LexA-Swi5(T490A,S492A,S505A,471-513) in pLexA202+PL |
| M3466 | pGAD-C3, expresses Gal4 activation domain on YEp vector with LEU2 marker |
| M3913 | GAD-Pho2 in pGAD-C3 |