TABLE 4.

Effect of alanine substitution mutations at potential phosphorylation sites on reporter activation

Relevant genotype				β-Galactosidase activity (%)b
SWI5 gene	Amino acid substitution ata:
	T490	S492	S505	HO(site B)-lacZ	CTS1(46)-lacZ
Not present				5	2
Wild type				100	100
	A			138	88
		A		321	157
			A	138	114
	A	A		365	170
		A	A	402	171
	A		A	133	119
	A	A	A	392	207
			P	10	49

^aThe indicated threonine or serine residues were converted to alanine by site-directed mutagenesis and cloned into YCp plasmids with a HIS3 marker. The S505P mutation was isolated in the genetic screen. Plasmids pRS313, M3114, M3562, M3563, M3564, M3565, M3566, M3567, M3568, and M3631 were transformed into yeast strain DY4678 or DY4680 that contains the integrated HO(site B)-lacZ or CTS1(46)-lacZ reporter, respectively. Three independent transformants were grown in selective medium and assayed for β-galactosidase activity. 

^bβ-Galactosidase activity measurements are normalized as a percentage of that of wild type, and standard errors were less than 15%.