TABLE 5.

Summary of Pho2 interaction-defective SWI5* alleles^a

Class	SWI5* allele	In vitro DNA binding with Pho2	LacZ activity (%) of in vivo reporters		In vivo activation (%)			Two-hybrid interaction with Pho2 (%)
			HO(site B)	CTS1(46)	HO(a1)	Native HO	SIC1
WTb		Full	100	100	100	100	100	100
A	S497P	None	6	96	3	37	71	3
	Q498R	None	6	88	6	49	89	5
B	E482K	Reduced	7	88	12	135	74	14
	S483G	Reduced	12	115	10	150	77	20
	F485S	Reduced	9	100	6	65	89	12
C	R484G	Full	11	119	25	111	99	47
	R484S	Full	14	81	47	93	79	98
	V494A	Full	7	129	1	42	52	8
	S505P	Full	5	44	1	29	85	75

^aThe data on in vitro cooperative DNA binding with Pho2 is from Table 3, the data on in vivo activation of the HO(site B)-lacZ and CTS1(46)-lacZ reporters is from Fig. 1, the data on in vivo activation of the HO(a1), native HO, and SIC1 genes is from Fig. 2 and 3, and the data on two-hybrid interaction with Pho2 is from Fig. 6. See footnote to Table 3 and legends to Fig. 1, 2, 3, and 6 for details. 

^bWT, wild type.