Table 2.

MP characterization of each formulation showing the yield percentage when formulated by spray drying, diameter (nm), polydispersity index (PI), EE of cGAMP, and the cGAMP weight loading.

Particle	Fabrication Method	Polymer	Diameter (nm)	PI	Encapsulation Efficiency (%)	cGAMP Weight Loading (μg/mg particle)
Baseline	Spray dry	Ace-DEX	674.3 ± 16.9	0.290	112.0 ± 16.6	11.2 ± 1.7
Concentration			1,121.8 ± 57.4	0.328	110.5 ± 8.9	11.1 ± 0.9
Temperature			686.7 ± 15.7	0.258	105.8 ± 13.0	10.6 ± 1.3
Inlet Flowrate			640.0 ± 21.9	0.242	103.5 ± 2.6	10.3 ± 0.3
Q-Flow			731.4 ± 14.9	0.275	102.8 ± 10.9	10.3 ± 1.1
Electrospray	Electrospray		712.8 ± 107.7	0.291	116.6 ± 7.2	11.7 ± 0.7
Emulsion	Double Emulsion		308.0 ± 26.1	0.190	63.9 ± 1.9	6.4 ± 0.2
PLGA	Spray dry	PLGA	1,151.4 ± 178.5	0.275	114.4 ± 4.4	11.4 ± 0.4