Key resources table.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| CD45R (B220) PE-Cyanine7 (clone RA3-6B2) | Thermo Fisher Sci. | Cat# 25-0452-82 |
| CD45R (B220) biotin (clone RA3-6B2) | Thermo Fisher Sci. | Cat# 13-0452-82 |
| CD45 BUV395 (clone 30-F11) | BD Pharmingen | Cat# 565967 |
| CD3e APC (clone 145-2C11) | Thermo Fisher Sci. | Cat# 17-0031-82 |
| CD3e biotin (clone 145-2C11) | Thermo Fisher Sci. | Cat# 13-0031-82 |
| CD11b PE (clone M1/70) | Thermo Fisher Sci. | Cat# 12-0112-82 |
| CD11b biotin (clone M1/70) | Thermo Fisher Sci. | Cat# 13-0118-82 |
| CD16/32 A700 (clone 93) | Thermo Fisher Sci. | Cat# 56-0161-82 |
| CD19 biotin (clone 1D3) | Thermo Fisher Sci. | Cat# 13-0193-82 |
| CD41 PerCP eFluor 710 (clone MWReg30) | Thermo Fisher Sci. | Cat# 46-0411-82 |
| CD45 PE (clone 3D-F11) | Thermo Fisher Sci. | Cat# 12-0451-82 |
| CD48 FITC (clone HM48-1) | Biolegend | Cat# 11-0481-82 |
| CD105 APC (clone 120414) | Biolegend | Cat# MJ7/18 |
| CD106 FITC (clone 429) | Biolegend | Cat# 105705 |
| CD49d PE (clone 9C10) | Biolegend | Cat# 103705 |
| CD150 PE-Cyanine7 (clone TC15-12F12.2) | BioLegend | Cat# 115914 |
| CD117 (c-Kit) APC-eFluor 780 (clone 2B8) | Thermo Fisher Sci. | Cat# 47-1171-82 |
| CD117 (c-Kit) biotin (clone 2B8) | Thermo Fisher Sci. | Cat# 13-1171-85 |
| Ly-6G/Ly-6C biotin (clone RB6-8C5) | Thermo Fisher Sci. | Cat# 13-5931-82 |
| Ly-6G/Ly-6C eFluor 450 (clone RB6-8C5) | Thermo Fisher Sci. | Cat# 48-5931-82 |
| NK1.1 biotin (clone PK136) | Thermo Fisher Sci. | Cat# 13-5941-82 |
| Streptavidin eFluor 450 | Thermo Fisher Sci. | Cat# 48-4317-82 |
| Ly-6A/E (Sca-1) PE-Cyanine5 (clone D7) | Thermo Fisher Sci. | Cat# 15-5981-82 |
| Ter-119 biotin (clone TER119) | Thermo Fisher Sci. | Cat# MA5-17819 |
| CD45 PE-Cy7 (clone 30-F11) | eBioscience | Cat# 25-0451-82 |
| CD45 APC (clone 30-F11) | BioLegend | Cat# 103112 |
| CD45.1 FITC (clone A20) | eBioscience | Cat# 11-0453-81 |
| CD45.2 PE-Cy7 (clone 104) | eBioscience | Cat# 25-0454-80 |
| Ly6G AF700 (clone RB6-8C5) | eBioscience | Cat# 56-5931-82 |
| Ly6G APC (clone RB6-8C5) | Biolegend | Cat# 108411 |
| CD3 FITC (clone 17A2) | BD Pharmingen | Cat# 561798 |
| CD3 eF450 (clone 17A2) | eBioscience | Cat# 48-0032-80 |
| CD3 PE-Cy7 (clone 17A2) | Biolegend | Cat# 560591 |
| CD19 PE (clone eBio1D3) | BD Pharmingen | Cat# 557399 |
| CD19 APC (clone eBio1D3) | eBioscience | Cat# 17-0193-80 |
| CD19 FITC (clone 1D3/CD19) | Biolegend | Cat# 152404 |
| CD11b PerCP Cy5.5 (clone M1/70) | eBioscience | Cat# 45-0112-82 |
| Cd11b PB (clone M1/70) | Biolegend | Cat# 101224 |
| anti-H3 | Cell Signaling Tech. | Cat# 9715 |
| anti-H3K18ac | Cell Signaling Tech. | Cat# 9675 |
| anti-Stat3 | Cell Signaling Tech. | Cat# 9132 |
| anti-stat3-phospho (Tyr705) | Cell Signaling Tech. | Cat# 9131 |
| anti-cFos | Sigma Aldrich | Cat# F7799 |
| Anti-rabbit Alexa 594 | Thermo Fisher Sci. | Cat# A-11012 |
| rabbit anti-mouse G-csf | R&D | Cat# MAB414 |
| rabbit IgG isotype control | R&D | Cat# MAB5005 |
| Rat IgG2b isotype control clone LTF-2 | BioXcell | Cat# BE0090 |
| anti-mouse IL-6R clone 15A7 | BioXcell | Cat # BE0047 |
| Biological samples | ||
| Human DNA | University of Milano Bicocca | n/a |
| Human blood | University of Milano Bicocca | n/a |
| Chemicals, peptides, and recombinant proteins | ||
| A485 | Medchemexpress | Cat# HY-107455 |
| C646 | Medchemexpress | Cat# HY-13823 |
| SGC-CBP30 | Medchemexpress | Cat# HY-15862 |
| 5-FU | Medchemexpress | Cat# HY-90006 |
| Fingolimod | Medchemexpress | Cat# HY-11065 |
| Forskolin | Medchemexpress | Cat# HY-15371 |
| NU9056 | Tocris | Cat# 4903 |
| 6-OHDA | Medchemexpress | Cat# HY-B1081 |
| AMD3100 | Medchemexpress | Cat# HY-10046 |
| Firategrast | Medchemexpress | Cat# HY-14951 |
| Dmp696 | Medchemexpress | Cat# HY-1213 |
| Antalarmin HCl | Medchemexpress | Cat# HY-103377 |
| Mifepristone (RU486) | Medchemexpress | Cat# HY-13683 |
| Metyrapone | Medchemexpress | Cat# HY-B1232 |
| Scopolamine | Selleckchem | Cat# S9326 |
| Resifineratoxin | Alomone Labs | Cat #R-400 |
| Kappa bungarotoxin | Alomone Labs | Cat# B-300 |
| recombinant murine G-csf | Peprotech | Cat# 250-05 |
| recombinant Acth | R&D | Cat# 3492 |
| recombinant murine Cxcl12 | R&D | Cat# 460-SD |
| Critical commercial assays | ||
| mouse corticosterone ELISA | abcam | Cat# ab108821 |
| mouse Acth ELISA | abcam | Cat# ab263880 |
| mouse Cxcl12 ELISA | R&D | Cat# MCX120 |
| mouse G-csf ELISA | R&D | Cat# MCS00 |
| mouse Il1b ELISA | R&D | Cat#DY401 |
| mouse Tnfa ELISA | R&D | Cat#DY410 |
| T-PER buffer | Thermo Fisher Sci. | Cat# 78510 |
| BCA assay | Thermo Fisher Sci. | Cat# 23227 |
| TRIzol reagent | Thermo Fisher Sci. | Cat# 15596026 |
| Reliaprep RNA tissue minikit | Promega | Cat# Z6010 |
| RNAsin | Promega | Cat# N2511 |
| M-MLV RT | Promega | Cat# M1701 |
| GoTag qPCR system | Promega | Cat# A6001 |
| CD31 microbeads, mouse | Miltenyi Biotec | Cat# 130-097-418 |
| CD45 microbeads, mouse | Miltenyi Biotec | Cat# 130-052-301 |
| 7-AAD | Thermo Fisher Sci. | Cat# A1310 |
| Zombie NIR | Biolegend | Cat# 423105 |
| CountBright absolute counting beads | Thermo Fisher Sci. | Cat# C36950 |
| Experimental models: Cell lines | ||
| adrenocortical Y1 cells | Bornstein Lab, Technical University of Dresden | n/a |
| murine Bcr-Abl ALL cells | Pereira Lab, Yale University | n/a |
| murine bone marrow-derived macrophages | C57BL/6J mice | n/a |
| L929 cells | DSMZ | #ACC 2 |
| Experimental models: Organisms/strains | ||
| C57BL/6J | Janvier Labs, Yale University and Jackson Laboratories | n/a |
| B6.129S6-Nr3c1tm2.1Ljm/J (Nr3c1flox/flox) | Jackson Laboratories; via J. Tuckermann | strain #012914 |
| B6.129-Gt(ROSA)26Sortm1(cre/ERT2)Tyj/J (Rosa26CreERT2) | Jackson Laboratories | strain #008463 |
| C57BL/6-Tg(Vav1-NUP98/HOXD13)G2Apla/J | Jackson Laboratories | strain #010505 |
| B6.S6JL-Ptrprca Pepcb/BoyJ (B6 CD45.1) | Jackson Laboratories | strain #002014 |
| B6.129P2(SJL)-Myd88im1.1Defr/J | Jackson Laboratories | strain #009088 |
| YRS ST2/IL25/TSLPR TKO | R.Locksley | n/a |
| Adrb1 tm1Bkk Adrb2 tm1Bkk | Jackson Laboratories | strain #003810 |
| B6(Cg)-Ifnar1tm1.2Ees/J | Jackson Laboratories | strain #028288 |
| ADX and sham-operated mice | Jackson Laboratories | n/a |
| Listeria monocytogenes strain: 10403s | R. Medzhitov | n/a |
| Oligonucleotides | ||
|
Actb F: GATCTGGCACCACACCTTCT R: GGGGTGTTGAAGGTCTCAAA |
Merck | n/a |
|
Itga4 F: TCTGTGAGTAGGTGGAGGAGATT R: CGGAAGTGACAAATATGCTGCAA |
Merck | n/a |
|
Cxcr4 F: TACATCTGTGACCGCCTTTA R: GGCAAAGAAAGCTAGGATGA |
Merck | n/a |
|
Cxcl12 F: AGCCAACGTCAAGCATCTGA R: TCGGGTCAATGCACACTTGT |
Merck | n/a |
|
Crhr1 F: ACAAACAATGGCTACCGGGA R: AAAGCCGAGATGAGGTTCCA |
Merck | n/a |
|
cFos F: GTCCGGTTCCTTCTATGCAG R: TAAGTAGTGCAGCCCGGAGT |
Merck | n/a |
|
Mc1r F: TTGGTGCCTGTATGTGTCCA R: CACGATGCTGACACTTACCATC |
Merck | n/a |
|
Mc2r F: TGCAGCTGACCGTTACATCA R: AGCATCAAAGGGAACAGCGA |
Merck | n/a |
|
Mc3r F: TATCCACATGTTCCTCTTCGCC R: TTGAAATGGGCCGTGTAGCA |
Merck | n/a |
|
Mc4r F: TTTTACGCGCTCCAGTACCA R: AATGTGAAGCCTCGCCATCA |
Merck | n/a |
|
Mc5r F: AATCTTACCCTGAACGCCTCA R: ACATGGGTGAGTGCAGGTTT |
Merck | n/a |
| Software and algorithms | ||
| FlowJo v10 | FlowJo | www.flowjo.com |
| Prism V9 | Graphpad | www.graphpad.com |
| BioRender | BioRender | www.biorender.com |
| ImageJ | NIH | https://imagej.nih.gov/ij/download.html |
| Single Cell Portal | Broad Institute | https://singlecell.broadinstitute.org/single_cell |
| Tabula Muris | Tabula Muris Consortium | https://tabula-muris.ds.czbiohub.org |
| Primerblast | NIH | https://www.ncbi.nlm.nih.gov/tools/primer-blast/ |
| Primer3 | n/a | https://primer3.ut.ee |