KEY RESOURCES TABLE
| SOURCE | IDENTIFIER | |
|---|---|---|
| Antibodies | ||
| FMC63-anti-idiotype (Dylight650 labelled) | Dr. Laurence Cooper, MD Anderson Cancer Center | N/A |
| 1A7 anti-14G2a idiotype (Dylight650 labelled) | NCI Frederick and University of Texas M.D. Anderson Cancer | N/A |
| human Her2-Fc recombinant proteins (Dylight650 labelled) | R&D Systems | Cat#1129-ER-050 |
| Human CD95-BV421 (clone DX2) | BioLegend | Cat#305624; RRID: AB_2561830 |
| human CD4-APC-Cy7 (clone OKT4) | BioLegend | Cat#317418 |
| human CD8-PerCp-Cy5.5 (clone SK1) | BioLegend | Cat#344709 |
| human CD8-PE (clone SK1) | BioLegend | Cat#344706 |
| human CD4-PE (clone OKT4) | BioLegend | Cat#317410 |
| human TIM-3-BV510 (clone F38–2E2) | BioLegend | Cat#345030 |
| human CD39-FITC (clone A1) | BioLegend | Cat#328205 |
| human CD39-PE (clone A1) | BioLegend | Cat#328207 |
| human CD39-APC-Cy7 (clone A1) | BioLegend | Cat#328225 |
| HA.11 Epitope Tag- PE (clone 16B12) | eBioscience | Cat#901518 |
| human CD73-PE-Cy7 (clone AD2) | eBioscience | Cat#25-0739-42; RRID: AB_2573368 |
| human PD-1-PE-Cy7 (clone eBio J105) | eBioscience | Cat#25-9985-82; RRID: AB_10853805 |
| human LAG-3-PE (clone 3DS223H) | eBioscience | Cat#12-2239-42; RRID: AB_2572597 |
| human CD45RO-PE-Cy7 (clone UCHL1) | eBioscience | Cat#25-0457-42, RRID: AB_10718534 |
| human CD45-PerCp-Cy5.5 (clone HI30) | eBioscience | Cat#45-0459-42; RRID: AB_10717530 |
| human CCR7-PE (clone 3D12) | eBioscience | Cat#12-1979-42; RRID: AB_10670625 |
| human CD3-BUV496 (clone UCHT1) | BD | Cat#612940 |
| human LAG-3-BV421 (clone T47-530) | BD | Cat#565720; RRID: AB_2744330 |
| human CD45RA-FITC (clone HI100) | BD | Cat#561882; RRID: AB_395879 |
| human CD45RA-BV711 (clone HI100) | BD | Cat#563733; RRID: AB_2738392 |
| human CD62L-BV605 (clone DREG-56) | BD | Cat#562719; RRID: AB_2744441 |
| human CD73-BV510 (clone AD2) | BD | Cat#563198; RRID: AB_2738062 |
| human CD4-BUV395 (clone SK3) | BD | Cat#563552; RRID: AB_2738273 |
| Human CD8-BUV805 (clone SK1) | BD | Cat#612889; RRID: AB_2833078 |
| CD278 (clone DX29) | BD | Cat#557801 |
| CD226 (clone 11A8) | BioLegend | Cat#302839 |
| CD69 (clone FN50) | BioLegend | Cat#310939 |
| CD62L (clone DREG-56) | BioLegend | Cat#104443 |
| CD4 (clone RPT-T4) | Fluidigm | Cat#3145001B |
| CD8 (clone RPT-T8) | Fluidigm | Cat#3146001B |
| CD28 (clone CD28.2) | BioLegend | Cat#302937 |
| CD274 2(clone 9E.2A3) | Fluidigm | Cat#3148017B |
| CD45RO (clone UCHL1) | Fluidigm | Cat#3149001B |
| CD134 (clone ACT35) | Fluidigm | Cat#3150023B |
| CD49 (clone P1E6-C5) | BioLegend | Cat#329502 |
| CD38 (clone HIT2) | BioLegend | Cat#303535 |
| TIM-3 (clone F38-2E2) | Fluidigm | Cat#3153008B |
| TGIT (clone MBSA43) | Fluidigm | Cat#3154016B |
| cPARP (clone F21-852) | BD | Cat#552597 |
| CD73 (clone AD2) | BioLegend | Cat#344002 |
| CD25 (clone M-A251) | BioLegend | Cat#356102 |
| CD137 (clone 4B4-1) | Fluidigm | Cat#3158013B |
| LAP (clone TW4-6H10) | BioLegend | Cat#349702 |
| IL-10 (clone JES3-9D7 | Biolegend | Cat#501423 |
| Tbet (clone 4B10) | Fluidigm | Cat#3160010B |
| CD152 (clone 14D3) | Fluidigm | Cat#3161004B |
| Foxp3 (clone PCH101) | Invitrogen | Cat#14-4776-82 |
| CD272 (clone MIH26) | Fluidigm | Cat#3163009B |
| CD39 (clone A1) | BioLegend | Cat#328221 |
| CD223 (clone 874501) | Fluidigm | Cat#3165037B |
| CD101 (clone G043H7 | Fluidigm | Cat#14-1019-82 |
| CD197 (clone G043H7) | Fluidigm | Cat#3167009A |
| CD127 (clone A019D5) | Fluidigm | Cat#3168017B |
| CD45RA (clone HI100) | Fluidigm | Cat#3170010B |
| Ki67 (clone Ki67) | Biolegend | Cat#350523 |
| CD122 (clone TU27) | Biolegend | Cat#339015 |
| CD304 (clone 12C2) | Biolegend | Cat#305631 |
| CD279 (clone EH12.2H7) | Fluidigm | Cat#3174020B |
| Helios (clone 22F6) | BioLegend | Cat#682602 |
| EOMES (clone WD1928) | Biolegend | Cat#14-4877-82 |
| TCF1 (clone C63D9) | Cell Signaling | Cat#2203S |
| CD27 (clone 323) | BioLegend | Cat#302802 |
| CD95 (clone 305631) | BioLegend | Cat#305631 |
| TOX (clone 6E6D03) | BioLegend | Cat#682602 |
| LEF1 (clone C12A5) | Cell Signaling | Cat#2230S |
| PKM2 (clone D78A4) | Cell Signaling | Cat#4053S |
| PKM1 | Thermo | Cat#15821-1-AP |
| BCAT1 (clone OTI3F5) | Thermo | Cat#CF504360 |
| BCAT2 (clone 7G3A11) | Novus | Cat#NBP2-61699 |
| ENO1 (clone EPR10863(B)) | Abcam | Cat#ab206120 |
| SDHA (clone 2E3GC12FB2AE2) | Abcam | Cat#ab14715 |
| CS (clone EPR8067) | Abcam | Cat#ab233838 |
| IDH1 (clone MAB7049) | R&D | Cat#843219 |
| IDH2 (clone EPR7577) | Abcam | Cat#ab230796 |
| GLS | LS BIO | Cat#LS-C166517-200 |
| Glut1 (clone EPR3915) | Abcam | Cat#ab252403 |
| GPT2 | Proteintech | Cat#16757-1-AP |
| ATPA5 (clone 15H4C4) | Abcam | Cat#ab14748 |
| NDUFB8 (clone 20E9DH10C12) | Thermo | Cat#459210 |
| Bacterial and virus strains | ||
| Stellar Competent Cells | Clontech, Takara | Cat# 636766 |
| Biological samples | ||
| Buffy Coats | Stanford Blood Bank | N/A |
| Whole Blood Samples of healthy donors | Stanford Blood Bank | N/A |
| Leukopaks from healthy donors | STEMCELL Technologies | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| Phusion Hot Start Flex 2X Master Mix | New England Biolabs | Cat# M0536L |
| Cisplatin | Fluidigm | Cat# 201064 |
| Fixable Viability Dye eFluorÔ 450 | Thermo Fisher | Cat# 65-0863-18 |
| Fixable Viability dye eFluor780 | Thermo Fisher | Cat# 65-0865-14 |
| DNA intercalator | Fluidigm | Cat# 201191B |
| Rapid DNA Ligation Kit | Roche | Cat# 11-635-379-001 |
| In-fusion HD Cloning System | Clontech, Takara | Cat# 639647 |
| ZymoPURE II Plasmid Maxiprep Kit | Zymo Research | |
| Dynabeads Human T-Expander CD3/CD28 | Thermo Fischer Scientific, Gibco | Cat# 11141D |
| Lipofectamine 2000 Transfection Reagent | Thermo Fischer Scientific | Cat# 11668500 |
| RetroNectin Recombinant HumanFibronectin Fragment | Takara | Cat# T100B |
| Dylight 650 Micoscale Antibody Labelling Kit | Thermo Fischer Scientific | Cat# 84536 |
| BD QuantiBRITE PE Beads | BD Biosciences | Cat# 340495 |
| Recombinant Human IL-2 | Preprotech | Cat# 200-02-1mg |
| Human IL-7 | Miltenyi Biotec | Cat# 130-095-367 |
| Human IL-15 | Miltenyi Biotec | Cat# 130-095-760 |
| Human Male AB Plasma-Derived Serum, Heat Inactivated (Human Thrombin) | Access Biologicals | Cat# 535-Hl |
| CryoStor CS10 | STEMCELL Technologies | Cat# 07930 |
| Alt-R.Sp. Cas9 protein | IDT | Cat# 1072532 |
| CPI444 | CORVUS BIOPHARMA | N/A |
| NECA | Torcis | Cat# 1691 |
| ATPlite Luminescence Assay System | PerkinElmer | Cat# 6016941 |
| Adenosine Assay Kit (Fluorometric) | Abcam | Cat# ab211094 |
| Ciclopirox (CPX) | Sigma-Aldrich | SML2011 |
| Critical commercial assays | ||
| RNEasy Plus mini-isolation | Qiagen | Cat# 74134 |
| CliniMACS CD8 GMP MicroBeads | Miltenyi Biotec | Cat# 170-076-703 |
| CliniMACS CD4 GMP MicroBeads | Miltenyi Biotec | Cat# 170-076-702 |
| RosetteSep Human T Cell Enrichment Cocktail | STEMCELL Technologies | Cat# 15061 |
| SepMate-50 Tubes | STEMCELL Technologies | Cat# 85450 |
| Human IFN-g ELISA MAX Deluxe | BioLegend | Cat# 430104 |
| Human IL-2 ELISA MAX Deluxe | BioLegend | Cat# 431804 |
| anti-PE MicroBeads | Miltenyi Biotec | Cat# 130-048-801 |
| APC BrDU kit | BD | Cat# 552598 |
| Annexin V Apoptosis Detection Kits | eBioscience | Cat# 88-8102-72 |
| Seahorse XF Cell Mito Stress Test Kit | Agilent Technologies | Cat# 103015-100 |
| Seahorse XF RPMI medium, without phenol red | Agilent Technologies | Cat# 103576-100 |
| Seahorse XF Glycolysis Stress Test Kit | Agilent Technologies | Cat# 103020-100 |
| P3 Primary Cell 4D-Nucleofector X Kit S | Lonza | Cat# V4XP-3032 |
| Cell-Tak | Corning | Cat# CB-40240 |
| Buffer PB | Qiagen | Cat# 19066 |
| MinElute PCR Purification Kit | Qiagen | Cat# 28004 |
| Index Kit 1 for Illumina | ApexBio | Cat# K1058 |
| CountBright Absolute Counting beads | ThermoFisher | Cat# C36950 |
| FACS Lysing Solution 10x | BD | Cat# 349202 |
| Deposited data | ||
| Bulk RNAseq data from CD4+ or CD8+ CD39+ vs CD39- | This paper | GSE250442 |
| Bulk RNAseq data from AAVS1KO, CD39KO, CD73KO, A2aR KO, ADA-OE HA-CAR T cells | This paper | GSE250442 |
| Bulk RNAseq data from HA-CAR T cells cultured in glucose- vs inosine-containing media | This paper | GSE250442 |
| ATACseq from HA-CAR T cells cultured in glucose- vs inosine-containing media | This paper | GSE250439 |
| Experimental models: Cell lines | ||
| 143B | ATCC | Cat# CRL-8303 |
| Nalm6 | Dr. David Barrett (Children’s Hospital of Philadelphia) | N/A |
| Nalm6-CD19low | Dr. Robbie Majzner (Dana Faber Cancer Insititute) | N/A |
| MG63.3 | Chand Khanna (National Cancer Institute, National Institutes of Health | N/A |
| Experimental models: Organisms/strains | ||
| Mice: NSG (NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ) | The Jackson Laboratory | Cat# JAX:005557; RRID:IMSR JAX:005557 |
| Oligonucleotides | ||
| AAVS1-GGGGCCACTAGGGACAGGAT | Synthego | N/A |
| ADORA2a-guide1-GUCUGUGGCCAUGCCCAUCA | Synthego | N/A |
| ADORA2a-guide2-UACACCGAGGAGCCCAUGAU | Synthego | N/A |
| CD73-guide1-GCGGGCGCCCGCGCGGCUCG | Synthego | N/A |
| CD73-guide2-CUAUGUGUCCCCGAGCCGCG | Synthego | N/A |
| CD39-UGGCACCCUGGAAGUCAAAG | Synthego | N/A |
| EIF5A-UACAUACAGGUCCAUCUGGU | Synthego | N/A |
| Recombinant DNA | ||
| RD 114 | previously used in our lab | N/A |
| CAR.CD19.FMC63.8aHTM.BBz | previously used in our lab | N/A |
| CAR.CD19.FMC63.28TM.28z | previously used in our lab | N/A |
| CAR.HER2.4D5.8aHTM.BBz | previously used in our lab | N/A |
| CAR.GD2.14g2a-E101K.28TM.28z | previously used in our lab | N/A |
| ADA1.HA. 8aHTM | This manuscript | N/A |
| Software and algorithms | ||
| FlowJo v10.7.1 | FlowJo, LLC | N/A |
| GraphPad Prism v8.4 | GraphPad Software Inc. | N/A |
| Living Image version (IVIS imaging) | Perkin Elmer | N/A |
| Biorender | Biorender | N/A |
| OMIQ | OMIQ | N/A |
| DESeq2 v1.30.1 | Ref# 70 | N/A |
| IGV v1.9.0 | Ref# 69 | N/A |
| ComBat_seq | Ref# 71 | N/A |
| chromVAR v1.12.0 | Ref# 72 | N/A |
| Seahorse Wave Pro | Agilent | N/A |
| OMIQ | OMIQ | N/A |
| Salmon | Salmon | salmon.html |
| SnapGene v5.2.3 | SnapGene v5.2.3 | N/A |
| Other | ||
| Incucyte ZOOM | Sartorius | |
| BD LSRFortessaÔ X-20 | BD Biosciences | |
| Beckman CytoFLEX analyzer | Beckman Coulter | |
| T Cell TransAct™, human | Miltenyi Biotec | Cat# 130-111-160 |
| Protamine sulfate | Frezenius-Cabi | N/A |