Table 3.
sMMO Structures
| M. capsulatus (Bath) MMOH (hydroxylase) | Resolution (Å) | PDB code |
|---|---|---|
| oxidized 4 °C | 2.20 | 1MMO |
| oxidized | 1.96 | 1FZ1 |
| oxidized | 1.70 | 1MTY |
| reduced in crystal | 2.15 | 1FYZ |
| anaerobically grown reduced | 2.40 | 1FZ5 |
| mixed valent, reduced in crystal | 2.15 | 1FZ2 |
| anaerobically grown mixed valent | 2.07 | 1FZ0 |
| methanol soaked | 2.05 | 1FZ6 |
| ethanol soaked | 1.96 | 1FZ7 |
| Xe pressurized | 3.30 | 1FZI |
| Xe pressurized | 2.60 | 1FZH |
| dibromomethane grown | 2.10 | 1FZ8 |
| iodoethane grown | 2.30 | 1FZ9 |
| pH 8.5 soaked | 2.38 | 1FZ4 |
| pH 6.2 soaked | 2.03 | 1FZ3 |
| Mn(II) soaked | 2.32 | 1XMF |
| apo (metal free) | 2.10 | 1XMG |
| Co(II) reconstituted | 2.32 | 1XMH |
| phenol soaked | 1.96 | 1XU5 |
| 6-bromohexanol soaked | 1.80 | 1XVB |
| 8-bromooctanol soaked | 2.00 | 1XVC |
| 4-fluorophenol soaked | 2.30 | 1XVD |
| 3-bromo-3-butenol soaked | 2.40 | 1XVE |
| chloropropanol soaked | 2.00 | 1XVF |
| bromoethanol soaked | 1.96 | 1XVG |
| bromophenol soaked | 2.30 | 1XU3 |
| cryoEM structure using graphene | 2.40 | 7TC8 |
| cryoEM structure using quantifoil | 2.90 | 7TC9 |
| M. trichosporium OB3b MMOH (hydroxylase) | Resolution (Å) | PDB code |
| oxidized | 2.00 | 1MHY |
| oxidized | 2.70 | 1MHZ |
| oxidized | 1.52 | 6VK6 |
| reduced in crystal | 2.12 | 6VK7 |
| MMOB, MMOR, and protein-protein complexes | Resolution (Å) | PDB code |
|---|---|---|
| M. capsulatus (Bath) MMOB NMR | 1CKV | |
| M. trichosporium OB3b MMOB NMR | 2MOB | |
| M. capsulatus (Bath) MMOR [2Fe-2S] domain NMR | 1JQ4 | |
| M. capsulatus (Bath) MMOR FAD/NADH binding domain NMR | 1TVC | |
| M. sporium MMOR FAD/NADH binding domain | 1.50 | 6L2U |
| M. capsulatus (Bath) MMOH-MMOB complex | 2.90 | 4GAM |
| M. trichosporium OB3b MMOH-MMOB with benzoate | 1.86 | 6VK5 |
| M. trichosporium OB3b MMOH-MMOB with succinate | 2.03 | 6VK8 |
| M. trichosporium OB3b MMOH-MMOB with one site reduced | 2.35 | 6VK4 |
| M. trichosporium OB3b MMOH-MMOB 5FW | 2.80 | 7M8Q |
| M. trichosporium OB3b MMOH-MMOB BTFA/K15C/5FW | 2.20 | 7M8R |
| M. trichosporium OB3b MMOH-MMOB S109A/T111A form 1 | 1.96 | 7S6Q |
| M. trichosporium OB3b MMOH-MMOB S109A/T111A form 2 | 2.40 | 7S7H |
| M. trichosporium OB3b MMOH-MMOB H5A | 1.89 | 7S6R |
| M. trichosporium OB3b MMOH-MMOB N107G/S110A | 1.98 | 7S6S |
| M. trichosporium OB3b MMOH-MMOB H33A | 1.82 | 7S6T |
| M. trichosporium OB3b diferric MMOH-MMOB XFEL | 1.95 | 6YD0 |
| M. trichosporium OB3b diferrous MMOH-MMOB XFEL | 1.95 | 6YDI |
| M. trichosporium OB3b reoxidized MMOH-MMOB XFEL | 1.95 | 6YDU |
| M. trichosporium OB3b diferrous MMOH-MMOB t = 0 XFELa | 2.00 | 6YY3 |
| M. sporium MMOH-MMOD | 2.60 | 67DK |
a
Treated the same as 6YDU but exposed to helium rather than O2.