TABLE 1.
Analysis of INK4a and ARF expression in cell lines by RT-PCRa
| Tissue source | Cell line | 5-Aza-CdR treatment | Expression of:
|
|
|---|---|---|---|---|
| ARF | INK4a | |||
| Colon | HCT116 | − | + | + |
| + | + | + | ||
| SW48 | − | + | − | |
| + | + | + | ||
| HCT15 | − | − | − | |
| + | + | + | ||
| LoVo | − | − | − | |
| SW837 | − | + | − | |
| HT-29 | − | + | − | |
| Cervix | C-33A | − | + | + |
| Bladder | 5637 | − | + | + |
| T24 | − | + | − | |
| + | + | + | ||
| J82 | − | + | + | |
| Lymphoid | Raji | − | + | − |
| + | + | + | ||
| CA46 | − | + | − | |
| HL-60 | − | + | − | |
| Liver | Hep 3B | − | + | + |
| Pancreas | CFPac-1 | − | + | − |
| + | + | + | ||
a
Expression of the transcript indicated was scored as present (+) or absent (−) after Southern hybridization with oligonucleotide probes specific for the first exon of each gene. 5-Aza-CdR treatments were at 1.0 μM for 72 h.