FIG. 4.

Analysis of RAR transcripts in RARγ₁ antisense transgene-transfected cells. (Left panel) Total RNAs (20 μg) from control (lane 1) and antisense transgene-transfected (lane 2) cells were analyzed by Northern blot hybridization to the BamHI insert of RARγ₁ cDNA. Two bands of the correct size (3.3 and 0.167 kb, respectively) can be visualized in transfected cells. (Right panel) RT-PCR for RAR expression in transfected cells grown in regular medium (C) or in the presence of 10 nM RA for 24 h. From left to right are RARα₁, -β₂ -γ₁, and -γ₂. Note that RARβ₂ mRNA is present independent of RA addition. The left lane contains molecular size markers (φX174 RFDNA/HaeIII fragments [GIBCO]).