Table 2. Comparison of sensitivities of direct peptide mapping, LC-MALDI, and LC-MS/MS(QE) for the analysis of GEE-labeled apo-calmodulin.
The LC-MALDI uses a gradient of 48 min, whereas the gradient time for LC-MS/MS is 57 min.
| MALDI | LC- MALDI |
LC-MS/MS | |
|---|---|---|---|
| # of peptide with modification | 4 | 10 | 12 |
| Peptide sequence coverage | 26% | 74% | 78% |