Table 2.

Ammonium sulfate precipitation of protease from kinema.

Ammonium Sulfate Saturation (%)	Volume (ml)	Protease Activity (PA) (U/ml)	Protein (mg/ml)	Total PA (U)	Total Protein (mg)	Specific Activity) (U/mg)
Crude	10	0.532 ± 0.025	0.164 ± 0.006	5.318	1.640	3.243
0–20	2	0.038 ± 0.005	0.222 ± 0.000	0.076	0.443	0.171
20–30	2	0.100 ± 0.008	0.113 ± 0.032	0.201	0.225	0.892
30–40	2	0.512 ± 0.013	0.076 ± 0.001	1.025	0.151	6.765
40–50	2	0.624 ± 0.008	0.051 ± 0.004	1.248	0.102	12.269
50–60	2	0.614 ± 0.003	0.101 ± 0.002	1.227	0.202	6.066
60–70 %	2	0.619 ± 0.042	0.081 ± 0.001	1.237	0.162	7.650
70–80%	2	0.094 ± 0.005	0.039 ± 0.006	0.187	0.078	2.390
80% (supernatant)	14	0.022 ± 0.026	0.026 ± 0.001	0.315	0.364	0.865