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Partial purification of protease from kinema.
| Purification Steps |
Total Volume (ml) |
Protease activity (U/ml) | Protein (mg/ml) | Total Activity (U) | Total Protein (mg) |
Specific Activity (U/mg) |
Purification fold | Yield % |
|---|---|---|---|---|---|---|---|---|
| Crude | 100 | 0.508 | 0.158 | 50.791 | 15.770 | 3.22 | 1.00 | 100.00 |
| 30–70% Amm. sulf. |
15 | 1.939 | 0.377 | 29.092 | 5.651 | 5.15 | 1.60 | 57.28 |
| Dialysed | 18.5 | 1.735 | 0.220 | 32.105 | 4.062 | 7.90 | 2.45 | 63.21 |
