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. 2024 Mar 8;14:5757. doi: 10.1038/s41598-024-56135-w

Table 5.

Gross pathological findings of NHP tissues from animals with TBI or PBI and treated with GT3 or vehicle.

Radiation Treatment Euthanasia (day) NHP# Sex Age (years) Gross pathological findings
PBI Vehicle 4 1608024 F 3.5 Overall, a supralethal dose of radiation caused significant injury to the organ system, inflammatory response manifested in the lung, animal observed to be 10% dehydrated, moderate generalized injected vasculature of the lung tissue, multifocal, raised lesions along the dorsal surface of the lungs, no redness associated with lesions observed
1603151 M 3.9 Inflammatory response manifested in the lung, both lungs observed to have few lesions, but no redness, animals appeared to be euhydrated, all other organs and tissues found to be within normal limits of size, shape, and consistency
1603060 F 3.9 A few lesions noted in both lungs, animal appeared to be euhydrated, moderate multifocal hemorrhagic congestion in the right middle lung lobe
7 1607024 F 3.6 Inflammatory response manifested in the lung, dehydration consistent with > 12 – 15% weight loss, mild to moderate generalized injected vasculature concentrated at the dorsal surface of the lung tissue, microsplenia (shriveled spleen), stomach full of straw-colored liquid content with petechial hemorrhage on the gastric walls noticed
1604091 M 3.8 Inflammatory response manifested in the lung, dehydration consistent with > 12 – 15% weight loss, several ulcerative lesions noted in both lungs, copious amount of pericardial fluid present around the heart, bruising and hemorrhages noted all over body, GI tract presented with significant petechiae in several regions
1605536 F 3.8 Dehydration consistent with > 15% weight loss, animal presented with sunken eyes, pale skin and dry mucus membranes, shriveled heart due to possible decreased pericardial fluid
1603103 M 3.9 Inflammatory response manifested in the lung, dehydration consistent with > 10 – 15% weight loss, pale skin, bruising, and dry mucus membranes, moderate generalized hemorrhagic congestion throughout the lung tissue, focal, pale lesion present on the surface of the liver, gas-distended stomach, petechiae and hemorrhage noted throughout GI tract
1603155 M 3.9 Gastrointestinal findings consistent with poor appetite, focal liver congestion noted to be a postmortem change, dehydration consistent with > 10 – 15% weight loss, animal presented with sunken eyes, pale skin, bruising, non-healing wounds, and dry mucus membranes, stomach empty, shriveled and few intussusceptions noted in the small intestines, all other organs and tissues found to be within normal limits of size, shape, and consistency
GT3 4 1603080 F 3.9 Inflammatory response manifested in the lung and spleen, animal observed to be euhydrated, few generalized injected vasculature of the lung tissue, roughened edges of the splenic surface presented with dark color
1608009 F 3.5 Severe hemorrhagic congestion of the lungs, right cranial, middle, accessory lung lobes have moderate to severe hemorrhagic congestion, left cranial and caudal lung lobes have mild to moderate multifocal hemorrhagic congestion, all other organs and tissues found to be within normal limits of size, shape, and consistency, animal observed to be euhydrated
1603051 M 3.9 Inflammatory response manifested in the lung, dehydration consistent with > 10% weight loss, few lesions noted on both lungs with petechiae and hemorrhage
7 1606094 F 3.7 Inflammatory response manifested in the lung, mild to moderate lesions noted in both lungs, heart contained decreased pericardial fluid, few raised projections on the liver surface, shriveled spleen, GI tract presented with several petechiae and hemorrhage, and mild to moderate gas distension in the stomach, dehydration consistent with > 10—12% weight loss
1604041 M 3.8 Inflammatory response manifested in the lung, moderate generalized injected vasculature of the lung tissue, few lesions noted in upper and middle lobes of right lung, rough edges of the spleen, left kidney firm to touch, animal observed to be euhydrated
1603158 F 3.9 Dehydration consistent with > 10 – 15% weight loss, animal presented with sunken eyes, pale skin and mucus membranes, hyperinflation throughout the lung tissue with few lesions noted on both lungs
1603047 M 3.9 Poor appetite, moderate gas distended stomach and intussusception noted in small intestines, all other organs and tissues found to be within normal limits of size, shape, and consistency, dehydration consistent with > 10 – 15% weight loss
1606085 M 3.7 Both lungs pale and hyperinflated, and few lesions noted in right lung, gastrointestinal findings consistent with poor appetite, empty stomach, dry intestinal tract, and mucus membranes, dehydration consistent with > 20% weight loss, all other organs and tissues found to be within normal limits of size, shape, and consistency
TBI Vehicle 4 RA2619 F 5.0 Moderate redness noted in both lungs, roughened edges on the splenic surface, all other organs and tissues found to be within normal limits of size, shape, and consistency
RA2692 M 4.9 A few minute red specks noted on both lungs, all other organs and tissues found to be within normal limits of size, shape, and consistency
RA2892 M 3.7 All organs and tissues found to be within normal limits of size, shape, and consistency, obvious exposure to supralethal irradiation
7 RA2378 F 5.0 Severe hemorrhagic congestion of both lungs, few petechiae noted in jejunum and colon, roughened edges on the splenic surface, enlarged spleen, a couple of intussusceptions noted in the small intestines, petechiae and hemorrhage observed in lining of small intestines, dehydration consistent with > 10% weight loss
RA2931 M 4.2 Significant GI damage present with hemorrhage, petechiae, moderate bruising all over body, a few non-healing wounds, a few skin ulcers, gas noted in the stomach, mild dehydration, watery stool in large intestine, dehydration consistent with body weight loss
RA2922 F 4.2 Redness and bruising noted all over body, many skin ulcers possibly from non-healing wounds, moderate petechiae noted in both lungs, roughened surfaces of spleen and kidneys, severe hemorrhage and petechiae in entire GI tract, dehydration consistent with > 10% weight loss
RA3238 F 3.8 Several lesions noted on both lungs, pale kidneys, inflamed gall bladder with foul smelling, grainy bile, petechiae and hemorrhage noted in regions of the small intestines and colon; few blood clots noted in the stomach, shriveled bladder, dehydration consistent with > 10% weight loss
RA3291 F 3.9 Bruising and mild to moderate petechiae noted all over body, colon contained red tarry stools, a couple of intussusceptions noted in the small intestines, inflamed gallbladder with grainy, dark green bile, moderate dehydration noted in abdominal cavity, ulcerative lesions noted in the lungs, spleen, and kidneys
GT3 4 RA2599 M 3.7 All other organs and tissues found to be within normal limits of size, shape, and consistency, obvious sign of supralethal radiation exposure
RA2781 M 5.1 Minimal redness noted in both lungs, all other organs and tissues found to be within normal limits of size, shape, and consistency
RA2115 F 5.5 All other organs and tissues found to be within normal limits of size, shape, and consistency, dehydrated
7 RA2829 M 5.2 Moderate GI damage present with few hemorrhages, petechiae, mild bruising in some parts of the body, mild dehydration, red–black, tarry stools in large intestine, intussusception in colon
RA2626 F 5.2 Moderate hemorrhage noted inside stomach, up to duodenal junction, ileocecal junction contained mild to moderate hemorrhage, petechiae noted in entire GI tract, moderate dehydration noted throughout GI tissue, dehydration consistent with > 10% weight loss
RA2528 F 4.0 Several lesions noted on heart surface, both lungs, pale kidneys, slightly shriveled spleen, petechiae and hemorrhage noted in regions of the small intestines and colon; dehydration consistent with > 10% weight loss
RA3246 F 3.9 Notable lesions in the upper and middle portions of both lungs, petechiae and hemorrhage noted in regions of the small intestines and colon; dehydration consistent with > 10% weight loss
RA2762 F 5.0 Significant GI tract damage, lesions noted in lungs, notable petechiae and hemorrhage in the entire GI tract, intussusception in small regions of the small intestine, gas noted in the upper GI, mild dehydration, redness on the surface on the intestines, shriveled bladder, dehydration consistent with > 10% weight loss