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. 2024 Mar 8;15:2142. doi: 10.1038/s41467-024-46463-w

Fig. 10. Summary of the main findings.

Fig. 10

a, b In wild-type mouse CA1 PNs, dendritic mitochondria display a striking degree of compartmentalized morphology, being long and fused in the apical tufts (SLM) with progressive fragmentation and occupancy of a smaller volume of the dendritic segments in SR and SO respectively. c We demonstrate using loss-of-function as well as rescue experiments that this compartmentalization of dendritic mitochondria morphology in CA1 PNs in vivo requires (1) neuronal activity (blocked by neuronal hyperpolarization following over-expression of Kir2.1) or by reducing the number of presynaptic inputs from CA3 to SR and SO dendrites by ~50% (shRNA Lphn3) in vivo, (2) requires activity-dependent activation of AMPK mediated by Camkk2 and (3) requires the AMPK-dependent phosphorylation of the pro-fission Drp1 receptor Mff and the anti-fusion protein Mtfr1l though its ability to suppress the pro-fusion Opa1 protein. These results demonstrate that mitochondrial fusion dominates over fission in apical tuft dendrites (SLM) and that activity-dependent mitochondrial fission dominates over fusion in both SO and SR dendritic compartments. See Discussion for details.