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. 2023 Nov 28;31(3):292–308. doi: 10.1038/s41418-023-01241-x

Fig. 4. RTN3 was sufficient to induce LD biogenesis in cardiomyocytes.

Fig. 4

Representative fluorescence images indicating LDs labeled with Bodipy 493/503 and nucleus labeled with DAPI (a) and quantitative analysis of LDs area per cell (b) (n = 10 images each group), scale bar = 10 μm; Representative fluorescence images indicating FFA labeled with Bodipy 558/568 C12 and LDs labeled with Bodipy 493/503 (c) and quantitative analysis of localization of FFA with LDs quantified with Mandar overlap R value analysis (d) (n = 10 images each group), scale bar = 10 μm; e Time-lapse montage of LDs biogenesis in live cells, scale bar = 10 mm; f Quantitative analysis of LD area (n = 10 images each group). Data are expressed as Mean ± SEM. Differences are significant for *P < 0.05, **P < 0.01.