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. 2024 Mar 8;15:2137. doi: 10.1038/s41467-024-46458-7

Fig. 4. The translation of Uqcr11 and Uqcrb mediating cardiomyocyte proliferation is regulated by Nat10 in an ac4C-independent manner.

Fig. 4

a Ac4C RIP analysis of Uqcr11 and Uqcrb (n = 3 independent experiments). Two-tailed Student’s t test with Welch’s correction. b Nat10 RIP analysis of Uqcr11 and Uqcrb (n = 5 independent experiments). Two-tailed Mann–Whitney two-tailed test. **p < 0.01 vs. IgG. c The distribution of Uqcr11 and Uqcrb between the nucleus and cytoplasm (n = 3 independent experiments). d The mutant site of Nat10. e The ac4C dot blot analysis in cardiomyocytes with WT or mut Nat10 overexpression. f Representative western blots and averaged data showing increased Nat10 in Nat10 and mutant Nat10 overexpressed cardiomyocytes compared with vector (n = 4 independent experiments). one-way ANOVA followed by Tukey’s Multiple Comparison tests. **p < 0.01 vs. Vector. g Polysome profiling analysis of Uqcrb and Uqcr11 in mutant Nat10 overexpressed cardiomyocytes (n = 4 independent experiments). h The effects of Uqcrb, Uqcr11 and Atp5j2 on cardiomyocyte proliferation analyzed by the detection of EdU, pH3 and Ki67 (n = 6 independent experiments). Scale bar: 50 μm. White arrows point the positive cardiomyocytes. One-way ANOVA followed by Dunnett’s Multiple Comparison tests. *p < 0.05, ***p < 0.001 vs. NC siRNA. Data are presented as mean ± SEM. Source data are provided as a Source Data file. Exact p values are provided in the Source Data file.