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. 2024 Mar 8;15:2113. doi: 10.1038/s41467-024-46220-z

Fig. 4. Spatially-derived MacroSigs associate with COO DLBCL subclassifications.

Fig. 4

The associations of MacroSigs with clinical categories (i.e., COO, genetic subtypes) were evaluated through the Fisher exact test. The overlap ratio refers to the number of patients classified as both a certain MacroSig and COO category, divided by the total number of patients classified in that particular COO category. A MacroSig1 (GC) and MacroSig2 (IF) were enriched in DLBCL COO classifications in bulk RNA gene expression profiles of DLBCL patients across eight publicly available transcriptomic datasets (n = 4594, 8 datasets). B MacroSig5 (RLT) and MacroSig6 (DLBCL) were enriched in DLBCL COO classifications in the above-mentioned eight datasets. C MacroSig3 (LZ) and MacroSig4 (DZ) were not distinctly enriched in any COO category in the above-mentioned eight datasets. D All genes of each MacroSig, through their respective module scores, were projected onto the Monocyte/Macrophage and B cell subsets of DLBCL scRNA-seq datasets (Ye et al; n = 17). The violin plot depicts, for each patient, the percentage of B cells and macrophages expressing a given MacroSig (module score > 0.1) The median and quartile bands are depicted. P values were calculated by a paired t test (see also Supplementary Figs. 5 and 6). Source data are provided as a Source Data file. Germinal center B-cell like, GCB; activated B-cell like, ABC; unclassified, UNC; monocyte/macrophage, Mono/Mac.